Kunishima S, Hayashi K, Kobayashi S, Naoe T, Ohno R
Department of Central Clinical Laboratory, Meijo Hospital, Nagoya, Japan.
Clin Chem. 1991 Feb;37(2):169-72.
A new sandwich-type enzyme-linked immunosorbent assay for quantifying glycocalicin, a proteolytic fragment of platelet membrane glycoprotein Ib, is described. The assay is based on the use of two monoclonal antibodies raised against glycoprotein Ib and involves the avidin-biotin technique. The detection limit is 7 micrograms/L and the range of glycocalicin determined in plasma is 0.01 to 1 mg/L. Assay time is 2 h. The intra-assay CV ranged from 3.6% to 5.2%, the interassay CV from 5.4% to 8.0%. Analytical recovery of purified glycocalicin added to a plasma pool averaged 96%. In 36 healthy subjects, the mean glycocalicin concentration in plasma was 0.36 (SD 0.07) mg/L (2.7 nmol/L). We conclude that this assay is suitable for measuring glycocalicin in plasma and is also more sensitive and precise than the previously published immunoassays based on competitive binding assay.
本文描述了一种用于定量血小板膜糖蛋白 Ib 的蛋白水解片段糖萼素的新型夹心型酶联免疫吸附测定法。该测定法基于使用两种针对糖蛋白 Ib 产生的单克隆抗体，并涉及抗生物素蛋白-生物素技术。检测限为 7 微克/升，血浆中糖萼素的测定范围为 0.01 至 1 毫克/升。测定时间为 2 小时。批内变异系数范围为 3.6%至 5.2%，批间变异系数为 5.4%至 8.0%。添加到血浆库中的纯化糖萼素的分析回收率平均为 96%。在 36 名健康受试者中，血浆中糖萼素的平均浓度为 0.36（标准差 0.07）毫克/升（2.7 纳摩尔/升）。我们得出结论，该测定法适用于测量血浆中的糖萼素，并且比先前基于竞争性结合测定法发表的免疫测定法更灵敏、更精确。
