系统性红斑狼疮与C1q：一种用于测定血清中C1q水平的定量酶联免疫吸附测定法。
Systemic lupus erythematosus and C1q: A quantitative ELISA for determining C1q levels in serum.
作者信息
Dillon Skyler P, D'Souza Anil, Kurien Biji T, Scofield R Hal
机构信息
Oklahoma Medical Research Foundation, Oklahoma City, 73104, USA.
出版信息
Biotechnol J. 2009 Aug;4(8):1210-4. doi: 10.1002/biot.200800273.
Abstract
C1q is of interest in systemic lupus erythematosus (SLE) research due to deficiencies in its activity being associated with the disease. Current published protocols for measuring C1q vary greatly in their results and ease of reproducibility. Due to this, average C1q concentrations have been reported between 56 and 276 microg/mL in non-SLE serum. We present an improved method for quantifying C1q concentrations, which employs a sandwich ELISA. This method has improved precision, cost efficiency, up-scaling, reproducibility, and uses significantly lesser volumes of serum sample when compared to RID and other methods for quantifying C1q. We report an average concentration of 113 +/- 40 microg/mL for C1q in non-SLE serum. The assay designed here will be useful in the high-throughput measurement of serum C1q in SLE cases.
摘要
由于C1q活性缺陷与系统性红斑狼疮（SLE）相关，因此它在SLE研究中备受关注。目前已发表的测量C1q的方案在结果和可重复性方面差异很大。因此，非SLE血清中C1q的平均浓度报告在56至276微克/毫升之间。我们提出了一种改进的定量C1q浓度的方法，该方法采用夹心酶联免疫吸附测定法（ELISA）。与放射免疫扩散法（RID）和其他定量C1q的方法相比，该方法具有更高的精密度、成本效益、可扩展性、可重复性，并且使用的血清样本量显著更少。我们报告非SLE血清中C1q的平均浓度为113±40微克/毫升。这里设计的检测方法将有助于对SLE病例的血清C1q进行高通量测量。
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