Kunishima S, Tahara T, Kato T, Kobayashi S, Saito H, Naoe T
Department of Internal Medicine, Nagoya University Branch Hospital, Japan.
Eur J Haematol. 1996 Jul;57(1):68-71. doi: 10.1111/j.1600-0609.1996.tb00492.x.
Using enzyme-linked immunosorbent assays, we measured the concentrations of serum thrombopoietin (TPO) and plasma glycocalicin, a proteolytic fragment of platelet glycoprotien Ib alpha, in 13 patients with myelodysplastic syndrome (MDS), aplastic anaemia (AA) or idiopathic thrombocytopenic purpura (ITP). In the patients with AA or MDS, the TPO concentrations were remarkably increased, and their glycocalicin concentrations were decreased compared with the normal control individuals. In the patients with ITP, however, the TPO and glycocalicin levels were not changed as much as in the AA/MDS patients in spite of the same degree of thrombocytopenia. During immunosuppressive treatment of ITP patients, there was an inverse relationship between the level of TPO and the platelet count. Thus, measurements of TPO and glycocalicin levels are useful for the diagnosis of thrombocytopenia, and our results from ITP patients did not support the model which suggested the simple feedback regulation of TPO in thrombocytopenia.
我们采用酶联免疫吸附测定法，检测了13例骨髓增生异常综合征（MDS）、再生障碍性贫血（AA）或特发性血小板减少性紫癜（ITP）患者的血清血小板生成素（TPO）浓度以及血浆糖萼素（血小板糖蛋白Ibα的蛋白水解片段）浓度。在AA或MDS患者中，TPO浓度显著升高，与正常对照个体相比，其糖萼素浓度降低。然而，在ITP患者中，尽管血小板减少程度相同，但TPO和糖萼素水平的变化幅度不如AA/MDS患者。在ITP患者的免疫抑制治疗期间，TPO水平与血小板计数呈负相关。因此，检测TPO和糖萼素水平有助于血小板减少症的诊断，并且我们对ITP患者的研究结果不支持血小板减少症中TPO简单反馈调节的模型。
