Franzini C, Morelli A M, Cattozzo G
Central Laboratory, Ospedale di Rho, Milano, Italy.
Clin Chem. 1991 Feb;37(2):236-8.
In assessing interference from bilirubin, the use of a synthetic soluble derivative (ditaurobilirubin, DTB) is recommended as a surrogate for the natural conjugates (Bc). We compared the interference effect of unconjugated bilirubin (Bu), Bc, and DTB, using six mechanized methods for serum creatinine measurement. No significant interference was noted in methods that include removal of proteins or in an enzymatic method involving NADH oxidation. Heavy (negative) interference was observed in an alkaline picrate method, and in direct enzymatic methods based on hydrogen peroxide measurement: interference was always more pronounced in the presence of the two soluble derivatives (Bc and DTB), whose interference was of the same magnitude. These results point out the utility of testing for bilirubin interference by using soluble derivatives, in addition to Bu, and suggest the feasibility of using DTB as a surrogate for Bc for this purpose.
在评估胆红素的干扰时，建议使用合成可溶性衍生物（二牛磺胆红素，DTB）作为天然共轭物（Bc）的替代物。我们使用六种血清肌酐测量的自动化方法，比较了未结合胆红素（Bu）、Bc和DTB的干扰效果。在包括蛋白质去除的方法或涉及NADH氧化的酶法中未观察到明显干扰。在碱性苦味酸盐法和基于过氧化氢测量的直接酶法中观察到严重（负）干扰：在两种可溶性衍生物（Bc和DTB）存在的情况下，干扰总是更明显，其干扰程度相同。这些结果指出了除Bu外，使用可溶性衍生物检测胆红素干扰的实用性，并表明为此目的使用DTB作为Bc替代物的可行性。
