Rajs G, Mayer M
Department of Clinical Biochemistry, hadassah Hospital Mount Scopus, Jerusalem, Israel.
Clin Chem. 1992 Dec;38(12):2411-3.
Bilirubin causes underestimation of serum creatinine in the Jaffé alkaline picrate assay. We report an approach for preventing bilirubin interference by pretreating serum samples with peroxidase and H2O2. The dissociation of bilirubin from albumin and its subsequent oxidation markedly reduces the bilirubin interference and enables accurate determination of creatinine concentrations by the Jaffé reaction even in hyperbilirubinemic sera. Within-run CVs were 2.6%, 4.0%, and 3.8% at mean creatinine concentrations of 88, 165, and 349 mumol/L, respectively (n = 20). Day-to-day CVs were 4.0%, 6.3%, and 5.8% for mean creatinine concentrations of 87, 168, and 364 mumol/L, respectively (n = 12). Average recovery of creatinine added to serum in the presence of 600 mumol/L bilirubin was 97% (n = 15). This method requires only small serum volumes (70 microL) and is easily applicable to automated analyzers that can be programmed to add three reagents consecutively.
在Jaffé碱性苦味酸盐法中，胆红素会导致血清肌酐的测定值偏低。我们报告了一种通过用过氧化物酶和过氧化氢预处理血清样本以防止胆红素干扰的方法。胆红素从白蛋白上解离并随后被氧化，这显著降低了胆红素的干扰，即使在高胆红素血症血清中，也能通过Jaffé反应准确测定肌酐浓度。在肌酐平均浓度分别为88、165和349μmol/L时，批内变异系数分别为2.6%、4.0%和3.8%（n = 20）。在肌酐平均浓度分别为87、168和364μmol/L时，日间变异系数分别为4.0%、6.3%和5.8%（n = 12）。在存在600μmol/L胆红素的情况下，添加到血清中的肌酐平均回收率为97%（n = 15）。该方法仅需要少量血清（70μL），并且易于应用于可通过编程连续添加三种试剂的自动分析仪。
