来自 Hall A 型肉毒梭菌的基因 CBO0515 编码罕见的酶 N5-(羧乙基)鸟氨酸合酶，EC 1.5.1.24。
The gene CBO0515 from Clostridium botulinum strain Hall A encodes the rare enzyme N5-(carboxyethyl) ornithine synthase, EC 1.5.1.24.
机构信息
Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
J Bacteriol. 2010 Feb;192(4):1151-5. doi: 10.1128/JB.01044-09. Epub 2009 Nov 20.
Abstract
Sequencing of the genome of Clostridium botulinum strain Hall A revealed a gene (CBO0515), whose putative amino acid sequence was suggestive of the rare enzyme N(5)-(1-carboxyethyl) ornithine synthase. To test this hypothesis, CBO0515 has been cloned, and the encoded polypeptide was purified and characterized. This unusual gene appears to be confined to proteolytic strains assigned to group 1 of C. botulinum.
摘要
对肉毒梭菌 Hall A 菌株基因组进行测序，发现了一个基因（CBO0515），其假定的氨基酸序列提示存在罕见的 N(5)-(1-羧乙基)鸟氨酸合酶。为了验证这一假设，克隆了 CBO0515，对其编码的多肽进行了纯化和鉴定。这个不寻常的基因似乎仅限于被归类为肉毒梭菌 1 组的蛋白水解菌株。
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本文引用的文献
1
Independent evolution of neurotoxin and flagellar genetic loci in proteolytic Clostridium botulinum.肉毒梭菌中神经毒素和鞭毛基因位点的独立进化
BMC Genomics. 2009 Mar 19;10:115. doi: 10.1186/1471-2164-10-115.
2
Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes.蛋白水解型（I 组）肉毒梭菌霍尔 A 菌株的基因组序列及梭菌基因组的比较分析
Genome Res. 2007 Jul;17(7):1082-92. doi: 10.1101/gr.6282807. Epub 2007 May 22.
3
Genetic diversity among Botulinum Neurotoxin-producing clostridial strains.产肉毒杆菌神经毒素的梭菌菌株间的遗传多样性。
J Bacteriol. 2007 Feb;189(3):818-32. doi: 10.1128/JB.01180-06. Epub 2006 Nov 17.
4
Phylogeny and taxonomy of the food-borne pathogen Clostridium botulinum and its neurotoxins.食源性病原体肉毒梭菌及其神经毒素的系统发育与分类学
J Appl Microbiol. 1998 Jan;84(1):5-17. doi: 10.1046/j.1365-2672.1997.00313.x.
5
Botulinum toxin as a biological weapon: medical and public health management.肉毒杆菌毒素作为生物武器：医学与公共卫生管理
JAMA. 2001 Feb 28;285(8):1059-70. doi: 10.1001/jama.285.8.1059.
6
Analysis of the structure and substrate binding of Phormidium lapideum alanine dehydrogenase.嗜石席藻丙氨酸脱氢酶的结构与底物结合分析。
Nat Struct Biol. 1998 Jul;5(7):561-7. doi: 10.1038/817.
7
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
8
Carboxyethyllysine in a protein: native carbonyl reductase/NADP(+)-dependent prostaglandin dehydrogenase.蛋白质中的羧乙基赖氨酸：天然羰基还原酶/NADP(+)依赖性前列腺素脱氢酶。
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):502-6. doi: 10.1073/pnas.90.2.502.
9
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.CLUSTAL W：通过序列加权、位置特异性空位罚分和权重矩阵选择提高渐进多序列比对的灵敏度。
Nucleic Acids Res. 1994 Nov 11;22(22):4673-80. doi: 10.1093/nar/22.22.4673.
10
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J Biol Chem. 1995 May 19;270(20):12226-34. doi: 10.1074/jbc.270.20.12226.
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