来自 Hall A 型肉毒梭菌的基因 CBO0515 编码罕见的酶 N5-(羧乙基)鸟氨酸合酶，EC 1.5.1.24。
The gene CBO0515 from Clostridium botulinum strain Hall A encodes the rare enzyme N5-(carboxyethyl) ornithine synthase, EC 1.5.1.24.
机构信息
Microbial Biochemistry and Genetics Unit, Oral Infection and Immunity Branch, NIDCR, National Institutes of Health, Bethesda, MD 20892, USA.
出版信息
J Bacteriol. 2010 Feb;192(4):1151-5. doi: 10.1128/JB.01044-09. Epub 2009 Nov 20.
Abstract
Sequencing of the genome of Clostridium botulinum strain Hall A revealed a gene (CBO0515), whose putative amino acid sequence was suggestive of the rare enzyme N(5)-(1-carboxyethyl) ornithine synthase. To test this hypothesis, CBO0515 has been cloned, and the encoded polypeptide was purified and characterized. This unusual gene appears to be confined to proteolytic strains assigned to group 1 of C. botulinum.
摘要
对肉毒梭菌 Hall A 菌株基因组进行测序，发现了一个基因（CBO0515），其假定的氨基酸序列提示存在罕见的 N(5)-(1-羧乙基)鸟氨酸合酶。为了验证这一假设，克隆了 CBO0515，对其编码的多肽进行了纯化和鉴定。这个不寻常的基因似乎仅限于被归类为肉毒梭菌 1 组的蛋白水解菌株。
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