产毒丁酸梭菌菌株BL6340和E型肉毒梭菌菌株真冈所产生的肉毒杆菌毒素无毒成分编码基因的核苷酸序列相似性。

Similarity in nucleotide sequence of the gene encoding nontoxic component of botulinum toxin produced by toxigenic Clostridium butyricum strain BL6340 and Clostridium botulinum type E strain Mashike.

作者信息

Fujii N, Kimura K, Yokosawa N, Oguma K, Yashiki T, Takeshi K, Ohyama T, Isogai E, Isogai H

机构信息

Department of Microbiology, School of Medicine, Sapporo Medical University, Hokkaido, Japan.

出版信息

Microbiol Immunol. 1993;37(5):395-8. doi: 10.1111/j.1348-0421.1993.tb03227.x.

DOI:10.1111/j.1348-0421.1993.tb03227.x

PMID:8355622

Abstract

The complete nucleotide and deduced amino acid sequence of the nontoxic component of botulinum type E progenitor toxin is determined in recombinant plasmid pU9BUH containing about 6.0 kb HindIII fragment obtained from chromosomal DNA of Clostridium butyricum strain BL6340. The open reading frame (ORF) of this nontoxic component gene is composed of 3,486 nucleotide bases (1,162 amino acid residues). The molecular weight calculated from deduced amino acid residues is estimated 13,6810.1. The present study revealed that 33 nucleotide bases of 3,486 are different in the nontoxic component gene between C. butyricum strain BL6340 and C. botulinum type E strain Mashike. This corresponds to the difference of 17 amino acid residues in these nontoxic component.

摘要

在含有从丁酸梭菌BL6340菌株染色体DNA获得的约6.0 kb HindIII片段的重组质粒pU9BUH中，测定了E型肉毒杆菌祖毒素无毒成分的完整核苷酸和推导氨基酸序列。该无毒成分基因的开放阅读框（ORF）由3486个核苷酸碱基（1162个氨基酸残基）组成。根据推导的氨基酸残基计算的分子量估计为136810.1。本研究表明，丁酸梭菌BL6340菌株和E型肉毒杆菌真冈菌株的无毒成分基因中，3486个核苷酸碱基中有33个不同。这对应于这些无毒成分中17个氨基酸残基的差异。