Moriishi K, Syuto B, Saito M, Oguma K, Fujii N, Abe N, Naiki M
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Infect Immun. 1993 Dec;61(12):5309-14. doi: 10.1128/iai.61.12.5309-5314.1993.
We examined production of ADP-ribosyltransferase C3 in 11 strains of Clostridium botulinum type C and D and their nontoxigenic derivatives. Antisera to C3 proteins of type C organisms divided C3 proteins roughly into at least two groups, bearing no relation to their bacterial types. The C3 gene of type D strain South African was isolated from a toxigenic phage library, and the complete sequence of the C3 gene was determined. The C3 protein of type D strain South African had 98% homology to the C3 protein of type C strain 003-9 and 66% homology to that of type D strain 1873. These results indicate that there are two types of C3 protein in type D organisms, as there are in type C organisms.
我们检测了11株C型和D型肉毒梭菌及其无毒衍生物中ADP核糖基转移酶C3的产生情况。针对C型菌株C3蛋白的抗血清将C3蛋白大致分为至少两组，这与它们的细菌类型无关。从一个产毒噬菌体文库中分离出D型南非菌株的C3基因，并测定了C3基因的完整序列。D型南非菌株的C3蛋白与C型003 - 9菌株的C3蛋白有98%的同源性，与D型1873菌株的C3蛋白有66%的同源性。这些结果表明，D型菌株中存在两种类型的C3蛋白，C型菌株中也是如此。
