Contreras R, Rogiers R, Van de Voorde A, Fiers W
Cell. 1977 Oct;12(2):529-38. doi: 10.1016/0092-8674(77)90129-5.
The nucleotide sequence of the SV40 Hind E fragment has been determined mainly by the partial chemical degradation procedure of Maxam and Gilbert (1977). The sequence of the strand with the same polarity as the late messenger RNA shows only one open reading frame for translation. Considering that VP3 corresponds to the carbosyl terminal part of VP2, and considering various evidence which indicates that the SV40 Hind E segment is part of the amino acid sequence of VP2-VP3. It continues clockwise in Hind K, where it terminates with a UAA signal. The latter is located 110 nucleotides beyond the initiation signal for the major structural protein VP1 (Fiers et al., 1975; Van de Voorde et al., 1976). Hence this small overlapping region of the genome codes for the synthesis of three different proteins in two different reading frames. The deduced amino acid sequence covers a major part of the vp3 poly peptide, and the amino acid composition is in good agreement with published values (Greenaway and Levine, 1973).
SV40 Hind E片段的核苷酸序列主要通过Maxam和Gilbert（1977年）的部分化学降解方法测定。与晚期信使RNA具有相同极性的链的序列仅显示一个开放阅读框用于翻译。考虑到VP3对应于VP2的羧基末端部分，并考虑到各种证据表明SV40 Hind E片段是VP2 - VP3氨基酸序列的一部分。它在Hind K中顺时针延续，在那里它以UAA信号终止。后者位于主要结构蛋白VP1的起始信号之后110个核苷酸处（Fiers等人，1975年；Van de Voorde等人，1976年）。因此，基因组的这个小重叠区域在两个不同的阅读框中编码三种不同蛋白质的合成。推导的氨基酸序列覆盖了vp3多肽的大部分，并且氨基酸组成与已发表的值高度一致（Greenaway和Levine，1973年）。
