Varani J, Schuger L, Fligiel S E, Inman D R, Chakrabarty S
Department of Pathology, University of Michigan Medical School, Ann Arbor 48109.
Int J Cancer. 1991 Feb 1;47(3):421-5. doi: 10.1002/ijc.2910470319.
Cell lines derived from 13 different human colon adenocarcinomas were examined for production of fibronectin by ELISA and for cell-surface expression of fibronectin by indirect immunofluorescence. Two squamous epithelial cell lines obtained from tumors of the upper aerodigestive tract were used as controls. None of the 13 colon carcinoma lines produced detectable amounts of fibronectin or showed detectable cell-surface staining with anti-fibronectin. The 2 squamous epithelial cell lines, in contrast, produced large amounts of fibronectin which could be detected in the culture medium and bound to the substratum. The squamous carcinoma cells also stained brightly when examined in the viable state by immunofluorescence with anti-fibronectin. In addition to being studied for fibronectin production, each cell line was also examined for the ability to interact with exogenous fibronectin in an adhesion assay. None of the colon carcinoma cells were adherent to fibronectin-coated culture dishes while the 2 squamous carcinoma cells rapidly attached and spread on this substratum. These data suggest that cell lines derived from adenocarcinomas of the colon are deficient in production of fibronectin and in their ability to interact with exogenous fibronectin. In their degree of deficiency, the colon carcinoma cells are significantly different from several different types of human tumor cell. The failure of the colon carcinoma cells to synthesize detectable amounts of fibronectin endogenously or to interact with exogenous fibronectin may explain, in part, the low degree of adhesive interaction which these cells have for their substratum. This, in turn, may influence the in vitro and in vivo properties of colon carcinoma cells.
通过酶联免疫吸附测定（ELISA）检测了源自13种不同人类结肠腺癌的细胞系中纤连蛋白的产生情况，并通过间接免疫荧光检测了纤连蛋白的细胞表面表达。使用从上下呼吸道肿瘤中获得的2种鳞状上皮细胞系作为对照。13种结肠癌细胞系均未产生可检测量的纤连蛋白，也未显示出用抗纤连蛋白进行的可检测到的细胞表面染色。相比之下，这2种鳞状上皮细胞系产生了大量纤连蛋白，可在培养基中检测到并结合于底物。当用抗纤连蛋白进行免疫荧光检测处于存活状态的鳞状癌细胞时，它们也呈现明亮的染色。除了研究纤连蛋白的产生外，还通过黏附试验检测了每个细胞系与外源性纤连蛋白相互作用的能力。没有一种结肠癌细胞能黏附于纤连蛋白包被的培养皿，而2种鳞状癌细胞能迅速附着并铺展在这种底物上。这些数据表明，源自结肠腺癌的细胞系在纤连蛋白的产生及其与外源性纤连蛋白相互作用的能力方面存在缺陷。在缺陷程度上，结肠癌细胞与几种不同类型的人类肿瘤细胞有显著差异。结肠癌细胞不能内源性合成可检测量的纤连蛋白或与外源性纤连蛋白相互作用，这可能部分解释了这些细胞与其底物之间黏附相互作用程度较低的原因。反过来，这可能会影响结肠癌细胞的体外和体内特性。
