Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462, Martonvasar, P.O. Box 19, Hungary.
Genome. 2009 Sep;52(9):748-54. doi: 10.1139/g09-047.
The absence of chromosome 7D in the wheat-Thinopyrum ponticum partial amphiploid BE-1 was detected previously by multicolour genomic in situ hybridization, sequential FISH (fluorescence in situ hybridization) using repetitive DNA probes, and SSR marker analysis. In the present study the previous cytogenetic and SSR marker analyses were expanded to include 25 other SSR markers assigned to wheat chromosomes 7A and 7D to confirm the presence of a 7A.7D translocation and to specify its composition. An almost complete chromosome 7A and a short chromosome segment derived from the terminal region of 7DL were detected, confirming the presence of a terminal translocation involving the distal regions of 7AL and 7DL. In both cases the position of the translocation breakpoint was different from that of known deletion lines. The identification of the 7AL.7DL translocation and its breakpoint position provides a new physical landmark for future physical mapping studies, opening up the possibility of more precise localization of genes or molecular markers within the terminal regions of 7DL and 7AL.
先前通过多色基因组原位杂交、使用重复 DNA 探针的顺序荧光原位杂交（FISH）以及 SSR 标记分析，检测到小麦-粗山羊草部分双二倍体 BE-1 中缺失染色体 7D。本研究扩展了先前的细胞遗传学和 SSR 标记分析，包括 25 个其他分配给小麦染色体 7A 和 7D 的 SSR 标记，以确认 7A.7D 易位的存在并指定其组成。检测到几乎完整的染色体 7A 和来自 7DL 末端区域的短染色体片段，证实存在涉及 7AL 和 7DL 远缘区的末端易位。在这两种情况下，易位断点的位置都与已知缺失系不同。7AL.7DL 易位及其断点位置的鉴定为未来的物理图谱研究提供了一个新的物理标记，为在 7DL 和 7AL 的末端区域内更精确地定位基因或分子标记开辟了可能性。
