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从老鼠身上收集奶的方法。

Methods for collecting milk from mice.

机构信息

Department of Animal Science, University of California, Davis, 2145 Meyer Hall, One Shields Avenue, Davis, CA 95616, USA.

出版信息

J Mammary Gland Biol Neoplasia. 2009 Dec;14(4):397-400. doi: 10.1007/s10911-009-9158-0. Epub 2009 Nov 24.

DOI:10.1007/s10911-009-9158-0
PMID:19936987
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2789213/
Abstract

Mouse models offer unique opportunities to study mammary gland biology and lactation. Phenotypes within the mammary glands, especially those caused by genetic modification, often arise during lactation, and their study requires the collection of adequate volumes of milk. We describe two approaches for collecting milk from lactating mice. Both methods are inexpensive, are easy to use in the laboratory or classroom, are non-invasive, and yield adequate volumes of milk for subsequent analyses.

摘要

小鼠模型为研究乳腺生物学和泌乳提供了独特的机会。乳腺内的表型,特别是那些由基因修饰引起的表型,通常在泌乳期间出现,因此需要收集足够量的乳汁进行研究。我们描述了两种从泌乳小鼠中收集乳汁的方法。这两种方法都很便宜,易于在实验室或课堂上使用,是非侵入性的,可以为后续分析提供足够量的乳汁。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0864/2789213/dde705a42926/10911_2009_9158_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0864/2789213/dde705a42926/10911_2009_9158_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0864/2789213/dde705a42926/10911_2009_9158_Fig1_HTML.jpg

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J Physiol Pharmacol. 2009 Oct;60 Suppl 3:113-8.
2
Technical note: Milk composition in mice--methodological aspects and effects of mouse strain and lactation day.技术说明:小鼠的乳汁成分——方法学方面以及小鼠品系和泌乳天数的影响
J Dairy Sci. 2009 Feb;92(2):632-7. doi: 10.3168/jds.2008-1563.
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Mice raised on milk transgenically enriched with n-3 PUFA have increased brain docosahexaenoic acid.用富含n-3多不饱和脂肪酸的转基因牛奶饲养的小鼠,其大脑中的二十二碳六烯酸含量增加。
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母体低蛋白饮食导致新生大鼠突触素表达和乳脂肪酸谱的性别特异性差异。
J Nutr Sci. 2024 Oct 14;13:e64. doi: 10.1017/jns.2024.46. eCollection 2024.
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Nutrients. 2024 Sep 28;16(19):3289. doi: 10.3390/nu16193289.
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