Department of Gene Expression, Adam Mickiewicz University, Miedzychodzka 5, 60-371, Poznan, Poland.
Arch Virol. 2010 Feb;155(2):169-79. doi: 10.1007/s00705-009-0556-9. Epub 2009 Nov 25.
In some RNA viruses (e.g. in brome mosaic virus, BMV), the same factor (intra- or intermolecular hybridization between viral RNA molecules) is capable of inducing two different processes: RNA silencing and RNA recombination. To determine whether there is some interplay between these two phenomena, we have examined if the BMV-based recombination vector containing a plant-genome-derived sequence can function as a gene-silencing vector. Surprisingly, we found that neither dsRNA forming during the replication of the BMV-based vector nor highly structured regions of its genome were effective RNAi triggers. Only mutants carrying a sequence complementary to the target mRNA functioned as gene silencing vectors and were steadily maintained in the infected plant. The constructs containing a sense sequence or inverted repeats did not induce gene silencing but instead were eliminated from the plant cells.
在一些 RNA 病毒中（例如，在 Bromemosaic virus，BMV 中），相同的因子（病毒 RNA 分子之间的分子内或分子间杂交）能够诱导两种不同的过程：RNA 沉默和 RNA 重组。为了确定这两种现象之间是否存在相互作用，我们研究了是否基于 BMV 的重组载体中包含的植物基因组衍生序列可以作为基因沉默载体发挥作用。令人惊讶的是，我们发现，在基于 BMV 的载体复制过程中形成的双链 RNA 以及其基因组的高度结构化区域都不是有效的 RNAi 触发物。只有携带与靶 mRNA 互补序列的突变体能作为基因沉默载体发挥作用，并在感染的植物中稳定维持。含有正义序列或反向重复的构建体不能诱导基因沉默，反而会从植物细胞中被清除。
