Departament de Química Analítica, and Institut de Biomedicina (IBUB), Universitat de Barcelona, Martí i Franquès 1-11, ES-08028 Barcelona.
Chem Biodivers. 2009 Nov;6(11):1822-7. doi: 10.1002/cbdv.200900120.
In this article, we describe a recently developed capillary-electrophoresis method for the determination of acidity constants and compare it with other existing methods. The new method is based on the use of an internal standard (compound similar in nature and pKa value to the analyte), and offers several benefits, since it has all the advantages of capillary electrophoresis. In addition, it is very fast, because the exact measure of the pH of the separation electrolytes is not needed, and only a few electrophoretic runs are required to perform a pKa determination. The acidity constants of some monoprotic weak acids and bases were determined by this fast method, yielding a very good agreement with literature values.
在本文中，我们描述了一种最近开发的毛细管电泳法，用于测定酸度常数，并将其与其他现有方法进行比较。新方法基于使用内标（与分析物在性质和 pKa 值上相似的化合物），并且具有几个优点，因为它具有毛细管电泳的所有优点。此外，它非常快速，因为不需要精确测量分离电解质的 pH 值，并且只需要进行几次电泳运行即可进行 pKa 测定。通过这种快速方法测定了一些一元弱酸和弱碱的酸度常数，与文献值非常吻合。
