Marveeva N A, Vasilenko M Iu, Shakhovskiĭ A M, Kuchuk N V
Tsitol Genet. 2009 Mar-Apr;43(2):27-32.
Transgenic plants of lettuce Lactuca sativa L. cv. Eralash, Sniezinka, Rubinovoje kruzevo with genes coding synthesis of tuberculosis antigenes have been obtained by Agrobacterium-mediated transformation. Cotyledons of in vitro seedlings were used as the initial material for transformation with plasmids pCB063 (genes ESAT6, nptII) and pCB064 (genes ESAT6:AG85B(-TMD), nptII). PCR-analysis has shown the presence both selective and target genes in all plants analyzed. At the same time, the RT-PCR has shown that both the presence and the absence of a transcription of gene ESAT6 at a stable transcription of a gene nptII is possible.
通过农杆菌介导的转化获得了含有编码结核抗原合成基因的生菜（Lactuca sativa L. cv. Eralash、Sniezinka、Rubinovoje kruzevo）转基因植株。以体外培养幼苗的子叶作为初始材料，用质粒pCB063（ESAT6基因、nptII基因）和pCB064（ESAT6:AG85B(-TMD)基因、nptII基因）进行转化。PCR分析表明，在所有分析的植株中均存在选择基因和靶基因。同时，RT-PCR表明，在nptII基因稳定转录的情况下，ESAT6基因转录的存在和缺失都是可能的。
