Matveeva N A, Kishchenko E M, Shakhovskiĭ A M, Potrokhov A A, Kuchuk N V
Tsitol Genet. 2011 Sep-Oct;45(5):11-6.
Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon alpha2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants.
用结核抗原ESAT6和Ag85B基因或人干扰素α2b基因转化的菊苣毛状根再生出了转基因植株。植株再生依赖光照，且在不含生长调节剂的培养基上发生。DNA PCR和RT-PCR分析表明，所有根系和再生植株中均存在并表达了选择基因和靶基因。
