分析铜绿假单胞菌在小鼠模型中胃肠道黏膜定植和水平传播的获得情况。
Analysis of acquisition of Pseudomonas aeruginosa gastrointestinal mucosal colonization and horizontal transmission in a murine model.
机构信息
Channing Laboratory, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
J Infect Dis. 2010 Jan 1;201(1):71-80. doi: 10.1086/648616.
BACKGROUND
Laboratory systems to study bacterial transmission and mucosal colonization leading to infection have not been utilized.
METHODS
We determined whether transmission of various strains of Pseudomonas aeruginosa among individual mice could occur and investigated the properties of such strains in establishing gastrointestinal (GI) mucosal colonization as well as in disseminating systemically after induction of neutropenia.
RESULTS
P. aeruginosa isolates associated with epidemic spread among patients with cystic fibrosis (CF) readily established GI colonization at higher levels than did strains associated with acute infections in patients without CF, and they outcompeted these strains. Colonization was associated with resistance to bile salts. However, epidemic CF isolates did not disseminate after induction of neutropenia and did not induce as much mucosal pathology as did strains that were capable of disseminating.
CONCLUSION
Murine models can be used to study P. aeruginosa transmission and early colonization, and the properties of these strains associated with their known clinical behaviors are mimicked in this setting.
背景
用于研究细菌传播和导致感染的黏膜定植的实验室系统尚未被应用。
方法
我们确定了不同的铜绿假单胞菌菌株在个体小鼠之间是否能够发生传播，并研究了这些菌株在建立胃肠道（GI）黏膜定植以及在诱导中性粒细胞减少后系统性传播方面的特性。
结果
与囊性纤维化（CF）患者的流行传播相关的铜绿假单胞菌分离株比与无 CF 患者急性感染相关的菌株更容易在更高水平上建立 GI 定植，并且它们能够竞争定植。定植与对胆盐的耐药性有关。然而，流行的 CF 分离株在诱导中性粒细胞减少后不会传播，并且不会引起像能够传播的菌株那样多的黏膜病理学变化。
结论
可以使用小鼠模型来研究铜绿假单胞菌的传播和早期定植，并且该模型中模拟了与这些菌株已知临床行为相关的特性。
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