Fractal Systems Inc., Belleair Beach, FL 33786, USA.
Biosens Bioelectron. 2010 Feb 15;25(6):1389-94. doi: 10.1016/j.bios.2009.10.034. Epub 2009 Oct 31.
Using electrochemical impedance spectroscopy (EIS) for biosensing applications typically requires repetitive experiments. To address this need, we have designed a multispecific electrochemical array with eight individually addressable 2mm-diameter gold working electrodes for rapid biosensing data accumulation by EIS in the presence of redox agent. The array allows to incorporate multiple negative controls in the course of a single binding experiment, as well as to perform parallel identical experiments to improve reliability of detection. The array is fit with attached electrochemical cell with Ag/AgCl mini reference electrode and can be used to process macro samples of 0.5-1 ml or micro samples of 5 microl in a drop-wise fashion. Eight individual EIS measurements are completed in 15 min. The reported array is disposable, economical and is easy to use. Examples of array use for label-free genetic sensing of 2.7 kb-long target Yersinia pestis DNA and for protein sensing of Ricin Toxin Chain A (RTA) are presented. We suggest the reported array design as a tool for researchers in the area of EIS sensing.
电化学阻抗谱（EIS）在生物传感应用中通常需要重复实验。为了解决这一需求，我们设计了一种具有 8 个可单独寻址的 2mm 直径金工作电极的多特异性电化学阵列，用于在存在氧化还原试剂的情况下通过 EIS 快速进行生物传感数据积累。该阵列允许在单个结合实验过程中纳入多个阴性对照，以及进行平行相同的实验以提高检测的可靠性。该阵列与带有 Ag/AgCl 微型参比电极的附属电化学池配合使用，可用于处理 0.5-1ml 的宏观样品或 5 微升的微样品，以逐滴方式进行处理。8 个单独的 EIS 测量在 15 分钟内完成。所报道的阵列是一次性的、经济的且易于使用。展示了该阵列在用于检测 2.7kb 长鼠疫耶尔森氏菌 DNA 的无标记基因传感和用于检测蓖麻毒素 A 链（RTA）的蛋白质传感方面的应用示例。我们建议将所报道的阵列设计作为 EIS 传感领域研究人员的工具。
