College of Chemistry, Biology, and Material Science, East China Institute of Technology, Fuzhou, China.
J Am Soc Mass Spectrom. 2010 Feb;21(2):290-3. doi: 10.1016/j.jasms.2009.10.015. Epub 2009 Oct 29.
Without any sample pretreatment, effervescent beverage fluids were manually sprayed into the primary ion plume created by using a nanoelectrospray ionization source for direct ionization, and the analyte ions of interest were guided into an ion trap mass spectrometer for tandem mass analysis. Functional ingredients (e.g., vitamins, taurine, and caffeine, etc.) and spiked impurity (e.g., cocaine) in various beverages, such as Red Bull energy drink, Coco-cola, and Pepsi samples were rapidly identified within 1.5 s. The limit of detection was found to be 7-15 fg (S/N = 3) for cocaine in different samples using the characteristic fragment (m/z 150) observed in the MS(3) experiments. Typical relative standard deviation and recovery of this method were 6.9%-8.6% and 104%-108% for direct analysis of three actual samples, showing that nanoextractive electrospray ionization tandem mass spectrometry is a useful technique for fast screening cocaine presence in beverages.
无需任何样品前处理，将碳酸饮料手动喷射到使用纳喷雾电离源产生的初级离子云中，进行直接电离，将感兴趣的分析物离子引导到离子阱质谱仪中进行串联质谱分析。在 1.5 秒内，可快速鉴定红牛能量饮料、可口可乐和百事可乐等各种饮料中的功能成分（如维生素、牛磺酸和咖啡因等）和添加杂质（如可卡因）。在 MS(3)实验中观察到特征碎片（m/z 150），发现在不同样品中可卡因的检出限为 7-15 fg（S/N = 3）。该方法对三种实际样品的直接分析的典型相对标准偏差和回收率分别为 6.9%-8.6%和 104%-108%，表明纳电喷雾串联质谱是一种快速筛查饮料中可卡因的有用技术。
