产后血清硫氧还蛋白浓度的增加及其与 CD8 淋巴细胞的关系。
Postpartum increase of serum thioredoxin concentrations and the relation to CD8 lymphocytes.
机构信息
Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine, Osaka, Japan.
出版信息
Ann Clin Biochem. 2010 Jan;47(Pt 1):62-6. doi: 10.1258/acb.2009.009120. Epub 2009 Nov 25.
BACKGROUND
There are few data on oxidative stresses during and after pregnancy, although aggravation of autoimmune disease is implicated in oxidative stress and occurs frequently in the postpartum period. Thioredoxin (TRX) is a stress-inducible protein, and is used as a good biomarker for oxidative stress. To clarify the changes in the levels of oxidative stress during and after pregnancy, we examined serum TRX levels and the numbers of lymphocyte subsets.
METHODS
We measured serum TRX levels by enzyme-linked immunosorbent assay (ELISA), and neutrophils, lymphocytes, and CD4 and CD8 lymphocytes by flow cytometry in peripheral blood from 88 healthy pregnant women, 26 just after delivery women, 77 healthy postpartum women and 19 healthy non-pregnant women.
RESULTS
The serum levels of TRX did not change during pregnancy, but increased in four, seven and 10 months postpartum. Serum TRX levels were correlated with the percentages of neutrophils in normal non-pregnant women and women one month postpartum, and with those of CD8 lymphocytes in early pregnant women and women one and four months postpartum.
CONCLUSIONS
Oxidative stress increased in the postpartum period, and the levels at one and four months postpartum were related to CD8 lymphocytes.
背景
尽管自身免疫性疾病的恶化与氧化应激有关，并且在产后期间经常发生，但关于怀孕期间和之后的氧化应激的数据却很少。硫氧还蛋白（TRX）是一种应激诱导蛋白，可用作氧化应激的良好生物标志物。为了阐明怀孕期间和之后氧化应激水平的变化，我们检查了血清 TRX 水平和淋巴细胞亚群的数量。
方法
我们通过酶联免疫吸附测定（ELISA）测量了 88 名健康孕妇、26 名分娩后妇女、77 名健康产后妇女和 19 名健康未怀孕妇女外周血中的血清 TRX 水平，并通过流式细胞术测量了中性粒细胞、淋巴细胞和 CD4 和 CD8 淋巴细胞。
结果
怀孕期间血清 TRX 水平没有变化，但在产后四个、七个和十个月增加。血清 TRX 水平与正常未怀孕妇女和产后一个月妇女中性粒细胞的百分比以及早孕妇女和产后一个和四个月妇女 CD8 淋巴细胞的百分比相关。
结论
产后氧化应激增加，产后一个月和四个月的水平与 CD8 淋巴细胞有关。
Zotero 插件