Honma Hajime, Asano Ryoki, Obara Masahiko, Otawa Kenichi, Suyama Yoshihisa, Nakai Yutaka
Laboratory of Sustainable Environmental Biology, Graduate School of Agricultural Science, Tohoku University, Miyagi 989-6711, Japan.
J Gen Appl Microbiol. 2009 Oct;55(5):359-71. doi: 10.2323/jgam.55.359.
Bacterial populations in epilithic biofilms collected from two distinct oligotrophic rivers of Japan were studied using denaturing gradient gel electrophoresis (DGGE). PCR-DGGE of the 16S rRNA gene and subsequent sequencing analysis suggested that in freshwater biofilms, members of the Cytophaga-Flavobacterium-Bacteroides (CFB) group were the most dominant, followed by those of alpha, beta, gamma, and delta-Proteobacteria; Leptospiraceae; and unidentified bacteria. Members of the CFB group, alpha-Proteobacteria, and cyanobacteria/plastid DNA were also detected from the biofilms collected from the estuary site, but the species in these samples differed from those detected in biofilms in the freshwater areas of the rivers. A comparison between the determined sequences revealed that similar bacterial species existed in biofilms at different sites of a river, and identical species existed in biofilms of distinct rivers. The results suggested that bacterial species in biofilms found in the estuary were different from those found in the freshwater areas of the rivers; however, the common bacterial species were distributed in biofilms collected from not only different sites along the same river but also sites in distinct oligotrophic rivers.
利用变性梯度凝胶电泳（DGGE）对从日本两条不同的贫营养河流采集的附石生物膜中的细菌种群进行了研究。对16S rRNA基因进行PCR-DGGE及后续测序分析表明，在淡水生物膜中，噬纤维菌-黄杆菌-拟杆菌（CFB）菌群的成员最为占优势，其次是α、β、γ和δ-变形菌纲；钩端螺旋体科；以及未鉴定的细菌。从河口站点采集的生物膜中也检测到了CFB菌群、α-变形菌纲和蓝细菌/质体DNA的成员，但这些样本中的物种与在河流淡水区域生物膜中检测到的不同。对所测定序列的比较显示，同一条河流不同位点的生物膜中存在相似的细菌物种，不同河流的生物膜中存在相同的物种。结果表明，河口生物膜中的细菌物种与河流淡水区域的不同；然而，常见的细菌物种不仅分布在同一条河流不同位点采集的生物膜中，也分布在不同贫营养河流的位点。
