Department of Histology and Embryology, Third Military Medical University, Chongqing, Republic of China.
Cells Tissues Organs. 2010;191(4):289-300. doi: 10.1159/000260063. Epub 2009 Nov 19.
Engrailed-1 (En-1) is a transcription factor involved in the development of the midbrain/hindbrain during mouse early embryogenesis. Although En-1 is expressed from embryogenesis to adulthood, there has been no detailed description of its expression during late mouse embryonic development. Here we report the expression pattern of En-1 in the mouse embryo from E10.5 to the neonatal state. With immunohistochemistry we found that En-1 was expressed in the central nervous system (CNS) from E10.5 to the neonatal state, mostly restricted to the midbrain/hindbrain junction. Outside the CNS, En-1 is dynamically expressed in several neural crest-associated structures including the cranial mesenchyme, the mandibular arches, the vagus nerve, the dorsal root ganglia, the sympathetic ganglia, the somites, the heart and the cloaca. Additionally, we found that in the CNS, most of the En-1 was located in the nuclei, while outside the CNS, En-1 was mainly expressed in the cytoplasm. These findings provided additional evidence that En-1 may be involved in the development of neural crest cells.
Engrailed-1（En-1）是一种转录因子，参与小鼠早期胚胎发生过程中的中脑/后脑发育。尽管 En-1 在胚胎发生到成年期都有表达，但对于其在晚期小鼠胚胎发育过程中的表达还没有详细的描述。在这里，我们报告了 En-1 在从 E10.5 到新生状态的小鼠胚胎中的表达模式。通过免疫组织化学染色，我们发现 En-1 从 E10.5 到新生状态都在中枢神经系统（CNS）中表达，主要局限于中脑/后脑交界处。在 CNS 之外，En-1 在包括颅间质、下颌弓、迷走神经、背根神经节、交感神经节、体节、心脏和泄殖腔在内的几个神经嵴相关结构中动态表达。此外，我们发现 CNS 中的大多数 En-1 位于细胞核中，而在 CNS 之外，En-1 主要表达在细胞质中。这些发现为 En-1 可能参与神经嵴细胞的发育提供了额外的证据。
