Department of Botany, University of Granada, Granada, Spain.
Int Arch Allergy Immunol. 2010;152(1):49-57. doi: 10.1159/000260083. Epub 2009 Nov 24.
The Poaceae family is considered one of the main causes of pollen allergy in industrialized countries. The aim of this study is to establish the dynamics of the Poaceae allergens and determine their distribution in the different-sized particles in the atmosphere.
The air of Granada (southern Spain) was sampled during the pollination period of Poaceae using a cascade impactor and a Hirst-type volumetric collector simultaneously. The sampled airborne allergens were analyzed by indirect ELISA and field emission scanning electron microscopy. Airborne pollen was evaluated with the Spanish Aerobiological Network methodology.
Poaceae pollen and allergenic activity have parallel dynamics during the period of maximum pollination, which is reflected in the positive correlations between the 2 variables. In addition, the highest Lol p 1 concentrations were recorded in particle sizes lower than 3.3 mum (stage 4-F). The Spearman correlation test showed that airborne allergens are not dependent on meteorological factors, such as humidity, wind direction or sunshine, however, Lol p 1 allergen correlated positively with Poaceae pollen.
The results of the present study confirm that the Lol p 1 allergen is detected more frequently with pollutants than with coarse particles with similar dynamics and a positive correlation between airborne pollen and aeroallergens. Moreover, Lol p 1 is released in stable weather conditions without large changes in humidity or temperature.
禾本科植物被认为是工业化国家花粉过敏的主要原因之一。本研究旨在确定禾本科过敏原的动态变化，并确定其在大气不同粒径颗粒中的分布。
在禾本科植物授粉期，同时使用级联冲击器和赫氏体积收集器在西班牙南部格拉纳达采集空气样本。通过间接 ELISA 和场发射扫描电子显微镜分析采集到的空气过敏原。使用西班牙空气生物学网络方法评估空气花粉。
禾本科花粉和变应原活性在花粉大量授粉期间具有平行的动态变化，这反映在这两个变量之间存在正相关。此外，在粒径小于 3.3 微米（第 4-F 级）的颗粒中记录到最高的 Lol p 1 浓度。Spearman 相关检验表明，空气变应原不受湿度、风向或阳光等气象因素的影响，但 Lol p 1 变应原与禾本科花粉呈正相关。
本研究结果证实，与粗颗粒相比，Lol p 1 过敏原在污染物中更频繁地被检测到，并且具有相似动态的细颗粒之间存在正相关关系，以及空气花粉与气传过敏原之间存在正相关关系。此外，Lol p 1 在湿度或温度没有大幅变化的稳定天气条件下释放。
