Xu Jing, Tian Yong-Sheng, Peng Ri-He, Xiong Ai-Sheng, Zhu Bo, Jin Xiao-Fen, Gao Jian-Jie, Hou Xi-Lin, Yao Quan-Hong
Agro-Biotechnology Research Center, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China.
BMB Rep. 2009 Nov 30;42(11):752-7. doi: 10.5483/bmbrep.2009.42.11.752.
Copper is essential but toxic in excess for aerobic organisms. Yeast transcription factor ACE1 functions as a sensor for copper and an inducer for the transcription of CUP1. In addition, ACE1 can activate the transcription of superoxide dismutase gene (sod1) in response to copper. In this study, we introduced the yeast ACE1 into Arabidopsis and analyzed its function in plant. Under high copper stress, the transgenic plants over-expressing ACE1 showed higher survival rate than the wild-type. We also found that over-expression of ACE1 in Arabidopsis increased the activities of SOD and POD, which were beneficial to the cell in copper buffering. Excess copper would suppress the expression of chlorophyll biosynthetic genes in Arabidopsis, RT-PCR analysis revealed that over-expression of ACE1 decrease the suppression. Together, our results indicate that ACE1 may play an important role in response to copper stress in Arabidopsis.
铜对于需氧生物来说必不可少，但过量时则具有毒性。酵母转录因子ACE1作为铜的传感器以及CUP1转录的诱导剂发挥作用。此外，ACE1可响应铜而激活超氧化物歧化酶基因（sod1）的转录。在本研究中，我们将酵母ACE1导入拟南芥并分析其在植物中的功能。在高铜胁迫下，过表达ACE1的转基因植物比野生型表现出更高的存活率。我们还发现，拟南芥中ACE1的过表达增加了SOD和POD的活性，这有利于细胞进行铜缓冲。过量的铜会抑制拟南芥中叶绿素生物合成基因的表达，RT-PCR分析表明，ACE1的过表达减轻了这种抑制作用。总之，我们的结果表明ACE1可能在拟南芥对铜胁迫的响应中发挥重要作用。
