Clinical Biochemical Laboratory, San Maurizio Regional Hospital of Bolzano, Via L. Boehler 5, 39100 Bolzano, Italy.
Clin Biochem. 2010 Mar;43(4-5):483-4. doi: 10.1016/j.clinbiochem.2009.11.009. Epub 2009 Nov 26.
Ethylene diamine tetraacetic acid (EDTA) plasma is the only suitable specimen recommended by the manufacturers to be used in the determination of BNP. It appears crucial to evaluate if more conventional heparin plasma samples could be reliably used for BNP determination. Aim of this study was to evaluate the use of heparin plasma sample for BNP determination.
Venous blood from 42 consecutive patients admitted at the division of cardiology was collected in two test tubes, with K(2)-EDTA (Group 1) and lithium heparin with gel separator (Group 2) and analysed within 20 min of blood collection.
Statistical analysis showed a significant difference between Group 1 and Group 2 (p<0.0001). Sample collected in K(2)-EDTA showed a significant underestimation when compared to lithium-heparin.
Our data showed that BNP could not be dosed on different collection tubes without altering the results. In our experimental conditions, interestingly we found that BNP levels are significantly lower if measured in EDTA plasma.
乙二胺四乙酸（EDTA）血浆是制造商推荐用于测定 BNP 的唯一合适标本。评估肝素血浆样本是否可用于 BNP 测定似乎至关重要。本研究旨在评估肝素血浆样本用于 BNP 测定的情况。
连续收集心内科 42 例患者的静脉血，分别注入含 K(2)-EDTA（第 1 组）和含凝胶分离锂肝素（第 2 组）的两支试管中，采血后 20 min 内进行分析。
统计分析显示第 1 组和第 2 组之间存在显著差异（p<0.0001）。与锂肝素相比，K(2)-EDTA 组的样本明显低估。
我们的数据表明，如果不改变结果，BNP 不能在不同的采集管中进行定量。在我们的实验条件下，有趣的是，我们发现如果在 EDTA 血浆中测量，BNP 水平显著降低。
