Department of Analytical Chemistry, Medical University of Lublin, 6 Staszica St., 20-081 Lublin, Poland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 1;878(1):34-8. doi: 10.1016/j.jchromb.2009.11.014.
The aim of this study was to estimate and compare the contents of selected metals in 65 pathological (diagnosed nodular goitre) and 50 healthy human thyroid tissues (taken during autopsies). Ion chromatography (IC) preceded by microwave mineralization was applied for the first time for determination of cadmium, cobalt, copper, iron, manganese, and zinc in human thyroid samples. The study proved that the concentrations of Cu(2+), Mn(2+), Fe(3+), and Zn(2+) were significantly higher in the control group (healthy thyroids) in comparison with the studied group (nodular goitre) (p<0.05), whereas for Co(2+) the difference between two means of concentration (healthy vs pathological thyroids) was not significant statistically at 0.05 significance level. Measurement accuracy was verified by measurements of NIST standard reference material (1566a Oyster Tissue). Very good precision (RSD below 5%) and recoveries (above 90%) were evaluated.
本研究旨在评估并比较 65 例病理性（诊断为结节性甲状腺肿）和 50 例健康人甲状腺组织（尸检时采集）中选定金属元素的含量。首次应用微波消解前离子色谱法（IC）测定人甲状腺组织中的镉、钴、铜、铁、锰和锌。研究表明，与研究组（结节性甲状腺肿）相比，对照组（健康甲状腺）中 Cu(2+)、Mn(2+)、Fe(3+)和 Zn(2+)的浓度显著更高（p<0.05），而 Co(2+)在健康甲状腺与病变甲状腺之间的浓度均值差异在 0.05 显著水平上无统计学意义。通过对 NIST 标准参考物质（1566a 牡蛎组织）的测量来验证测量精度。评价结果表明，该方法具有非常好的精密度（RSD 低于 5%）和回收率（高于 90%）。
