Department of Molecular Diagnosis, Graduate School of Medicine, Chiba University, Japan.
Clin Chim Acta. 2010 Feb;411(3-4):285-90. doi: 10.1016/j.cca.2009.11.026. Epub 2009 Nov 27.
Fourier-transform infrared (FT-IR) spectroscopy is a simple and reagent-free physicochemical analysis method, and is a potential alternative to more time-consuming and labor-intensive procedures. In this study, we aimed to use FT-IR spectroscopy to determine serum concentrations of chylomicron-triglyceride (TG) and very low density lipoprotein (VLDL)-TG.
We analyzed a chylomicron fraction and VLDL fraction, which had been obtained by ultracentrifugation, to search for wavelengths to designate to each fraction. Then, partial least square (PLS) calibrations were developed using a training set of samples, for which TG concentrations had been determined by conventional procedures. Validation was conducted with another set of samples using the PLS model to predict serum TG concentrations on the basis of the samples' IR spectra. We analyzed a total of 150 samples.
Serum concentrations of chylomicron-TG and VLDL-TG estimated by FT-IR spectroscopy agreed well with those obtained by the reference method (r=0.97 for both lipoprotein fractions). FT-IR spectrometric analysis required 15mul of serum and was completed within 1min.
Serum chylomicron-TG and VLDL-TG concentrations can be determined with FT-IR spectroscopy. This rapid and simple test may have a great impact on the management of patients with dyslipidemia.
傅里叶变换红外（FT-IR）光谱是一种简单且无需试剂的物理化学分析方法，是更耗时、更繁琐的方法的潜在替代方法。本研究旨在使用 FT-IR 光谱法测定乳糜微粒甘油三酯（TG）和极低密度脂蛋白（VLDL）-TG 的血清浓度。
我们分析了超速离心得到的乳糜微粒部分和 VLDL 部分，以寻找可用于指定各部分的波长。然后，使用传统方法测定 TG 浓度的样本训练集建立偏最小二乘（PLS）校准。使用 PLS 模型对另一组样本进行验证，根据样本的红外光谱预测血清 TG 浓度。我们共分析了 150 个样本。
FT-IR 光谱法估计的血清乳糜微粒-TG 和 VLDL-TG 浓度与参考方法一致（两种脂蛋白部分的 r=0.97）。FT-IR 光谱分析需要 15μl 血清，在 1 分钟内完成。
FT-IR 光谱法可用于测定血清乳糜微粒-TG 和 VLDL-TG 浓度。这种快速简单的测试可能对血脂异常患者的管理产生重大影响。
