Wrocław University of Science and Technology, Faculty of Fundamental Problems of Technology, Department of Biomedical Engineering, 27 Stanisława Wyspiańskiego St., 50-370 Wrocław, Poland.
Jagiellonian University Medical College, Department of Analytical Biochemistry, 9 Medyczna St., 30-688 Kraków, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Aug 5;183:239-246. doi: 10.1016/j.saa.2017.04.020. Epub 2017 Apr 18.
Hypertriglyceridemia, related with triglyceride (TG) in plasma above 1.7mmol/L is one of the cardiovascular risk factors. Very low density lipoproteins (VLDL) are the main TG carriers. Despite being time consuming, demanding well-qualified staff and expensive instrumentation, ultracentrifugation technique still remains the gold standard for the VLDL isolation. Therefore faster and simpler method of VLDL-TG determination is needed. Vibrational spectroscopy, including FT-IR and Raman, is widely used technique in lipid and protein research. The aim of this study was assessment of Raman and FT-IR spectroscopy in determination of VLDL-TG directly in serum with the isolation step omitted. TG concentration in serum and in ultracentrifugated VLDL fractions from 32 patients were measured with reference colorimetric method. FT-IR and Raman spectra of VLDL and serum samples were acquired. Partial least square (PLS) regression was used for calibration and leave-one-out cross validation. Our results confirmed possibility of reagent-free determination of VLDL-TG directly in serum with both Raman and FT-IR spectroscopy. Quantitative VLDL testing by FT-IR and/or Raman spectroscopy applied directly to maternal serum seems to be promising screening test to identify women with increased risk of adverse pregnancy outcomes and patient friendly method of choice based on ease of performance, accuracy and efficiency.
高甘油三酯血症，与血浆中甘油三酯（TG）超过 1.7mmol/L 有关，是心血管风险因素之一。极低密度脂蛋白（VLDL）是 TG 的主要载体。尽管超速离心技术耗时、需要高素质的工作人员和昂贵的仪器，但它仍然是 VLDL 分离的金标准。因此，需要更快、更简单的 VLDL-TG 测定方法。振动光谱学，包括傅里叶变换红外（FT-IR）和拉曼，是脂质和蛋白质研究中广泛使用的技术。本研究的目的是评估拉曼和 FT-IR 光谱法在省略分离步骤的情况下直接在血清中测定 VLDL-TG 的应用。用参考比色法测量了 32 例患者血清和超速离心 VLDL 级分中的 TG 浓度。获取了 VLDL 和血清样品的 FT-IR 和拉曼光谱。偏最小二乘（PLS）回归用于校准和留一法交叉验证。我们的结果证实了直接在血清中用拉曼和 FT-IR 光谱法进行无试剂测定 VLDL-TG 的可能性。FT-IR 和/或拉曼光谱法直接应用于母血清中的定量 VLDL 检测似乎是一种有前途的筛查试验，可以识别出有不良妊娠结局风险增加的女性，并且基于易用性、准确性和效率，是一种患者友好的选择方法。
