Division of Immunology, Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
Dev Comp Immunol. 2010 Apr;34(4):406-17. doi: 10.1016/j.dci.2009.11.009. Epub 2009 Dec 6.
The repertoire of gut associated T cells is shaped by exposure to microbes, including the natural enteric microflora. Previous studies compared the repertoire of gut associated T cell populations in germ free (GF) and conventional mammals often focussing on intra-epithelial lymphocyte compartments. Using GF, conventional and monocolonised (gnotobiotic) chickens and chicken TCRbeta-repertoire analysis techniques, we determined the influence of microbial status on global and regional enteric TCRbeta repertoires. The gut of conventionally reared chickens exhibited non-Gaussian distributions of CDR3-lengths with some shared over-represented peaks in neighbouring gut segments. Sequence analysis revealed local clonal over-representation. Germ-free chickens exhibited a polyclonal, non-selected population of T cells in the spleen and in the gut. In contrast, gnotobiotic chickens exhibited a biased repertoire with shared clones evident throughout the gut. These data indicate the dramatic influence of enteric microflora complexity on the profile of TCRbeta repertoire in the gut at local and global levels.
肠道相关 T 细胞的库是由暴露于微生物（包括天然肠内微生物群）形成的。先前的研究比较了无菌（GF）和常规哺乳动物中肠道相关 T 细胞群体的库，通常集中在上皮内淋巴细胞区室上。使用无菌、常规和单定植（无菌动物）鸡和鸡 TCRβ-库分析技术，我们确定了微生物状态对肠道 TCRβ-库的整体和局部的影响。常规饲养的鸡的肠道表现出 CDR3 长度的非高斯分布，在相邻肠道段中存在一些共同的过表达峰。序列分析显示局部克隆过表达。无菌鸡的脾脏和肠道中存在多克隆、未选择的 T 细胞群体。相比之下，无菌动物鸡表现出偏向性的库，整个肠道中都有共同的克隆。这些数据表明肠道内微生物群复杂性对肠道 TCRβ-库的局部和整体水平的显著影响。
