Englund G, Hoggan M D, Theodore T S, Martin M A
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Virology. 1991 Mar;181(1):150-7. doi: 10.1016/0042-6822(91)90479-u.
Three molecular clones of HIV-1, derived from a single isolate (AL1), exhibited distinct replicative and cytopathic properties during propagation in a human T cell line. The phenotypic differences observed were attributable, in large part, to changes affecting the viral LTR. Nucleotide sequence and PCR analyses demonstrated the presence of novel duplications or deletions involving the NF-kappa B motif. These changes in the enhancer element were identified in the original AL1 virus stock. Subcloning of the variant NF-kappa B segments into LTR-driven CAT expression vectors confirmed a correlation between promoter activity and replicative/cytopathic capacity.
从单个分离株（AL1）获得的三个HIV-1分子克隆，在人T细胞系中增殖期间表现出不同的复制和细胞病变特性。观察到的表型差异在很大程度上归因于影响病毒长末端重复序列（LTR）的变化。核苷酸序列和聚合酶链反应（PCR）分析表明存在涉及核因子κB（NF-κB）基序的新型重复或缺失。这些增强子元件的变化在原始AL1病毒株中被鉴定出来。将变异的NF-κB片段亚克隆到LTR驱动的氯霉素乙酰转移酶（CAT）表达载体中，证实了启动子活性与复制/细胞病变能力之间的相关性。
