Novitsky V A, Montano M A, McLane M F, Renjifo B, Vannberg F, Foley B T, Ndung'u T P, Rahman M, Makhema M J, Marlink R, Essex M
Harvard AIDS Institute, Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts 02115, USA.
J Virol. 1999 May;73(5):4427-32. doi: 10.1128/JVI.73.5.4427-4432.1999.
To better understand the virological aspect of the expanding AIDS epidemic in southern Africa, a set of 23 near-full-length clones of human immunodeficiency virus type 1 (HIV-1) representing eight AIDS patients from Botswana were sequenced and analyzed phylogenetically. All study viruses from Botswana belonged to HIV-1 subtype C. The interpatient diversity of the clones from Botswana was higher than among full-length isolates of subtype B or among a set of full-length HIV-1 genomes of subtype C from India (mean value of 9. 1% versus 6.5 and 4.3%, respectively; P < 0.0001 for both comparisons). Similar results were observed in all genes across the entire viral genome. We suggest that the high level of HIV-1 diversity might be a typical feature of the subtype C epidemic in southern Africa. The reason or reasons for this diversity are unclear, but may include an altered replication efficiency of HIV-1 subtype C and/or the multiple introduction of different subtype C viruses.
为了更好地了解南部非洲不断扩大的艾滋病流行的病毒学特征，对一组代表来自博茨瓦纳8名艾滋病患者的23个1型人类免疫缺陷病毒（HIV-1）近全长克隆进行了测序，并进行了系统发育分析。所有来自博茨瓦纳的研究病毒均属于HIV-1 C亚型。博茨瓦纳克隆的患者间多样性高于B亚型全长分离株或来自印度的一组C亚型HIV-1全长基因组（平均值分别为9.1%、6.5%和4.3%；两项比较的P均<0.0001）。在整个病毒基因组的所有基因中都观察到了类似的结果。我们认为，HIV-1的高度多样性可能是南部非洲C亚型流行的一个典型特征。这种多样性的原因尚不清楚，但可能包括HIV-1 C亚型复制效率的改变和/或不同C亚型病毒的多次引入。
