Jiangsu Key Laboratory of New Power Batteries, Jiangsu Key Laboratory of Biofunctional Materials, Laboratory of Electrochemistry, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, PR China.
Biosens Bioelectron. 2012 Jan 15;31(1):244-50. doi: 10.1016/j.bios.2011.10.026. Epub 2011 Nov 9.
Choline, as a marker of cholinergic activity in brain tissue, is very important in biological and clinical analysis, especially in the clinical detection of the neurodegenerative disorders disease. This work presents an electrochemical approach for the detection of choline based on prussian blue modified iron phosphate nanostructures (PB-FePO(4)). The obtained nanostructures showed a good catalysis toward the electroreduction of H(2)O(2), and an amperometric choline biosensor was developed by immobilizing choline oxidase on the PB-FePO(4) nanostructures. The biosensor exhibited a rapid response (ca. 2s), low detection limit (0.4±0.05 μM), wide linear range (2 μM to 3.2 mM), high sensitivity (~75.2 μAm M(-1) cm(-2)), as well as good stability and repeatability. In addition, the common interfering species, such as ascorbic acid, uric acid and 4-acetamidophenol did not cause obvious interference due to the low detection potential (-0.05 V versus saturated calomel electrode). This nanostructure could be used as a promise platform for the construction of other oxidase-based biosensors.
胆碱作为脑组织中胆碱能活性的标志物，在生物和临床分析中非常重要，特别是在神经退行性疾病的临床检测中。本工作提出了一种基于普鲁士蓝修饰的磷酸铁纳米结构（PB-FePO(4)）电化学检测胆碱的方法。所得纳米结构对 H(2)O(2)的电化学还原表现出良好的催化作用，并通过将胆碱氧化酶固定在 PB-FePO(4)纳米结构上，开发了一种安培型胆碱生物传感器。该生物传感器具有快速响应（约 2s）、低检测限（0.4±0.05 μM）、宽线性范围（2 μM 至 3.2 mM）、高灵敏度（~75.2 μAm M(-1) cm(-2)）以及良好的稳定性和重复性。此外，由于检测电位低（相对于饱和甘汞电极为-0.05 V），常见的干扰物质，如抗坏血酸、尿酸和 4-乙酰氨基酚，不会引起明显的干扰。这种纳米结构可以作为构建其他基于氧化酶的生物传感器的有前途的平台。
