稀酸与离子液体预处理柳枝稷的比较：生物量抗性、脱木质素和酶解糖化。

Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification.

机构信息

Joint BioEnergy Institute, Physical Biosciences Division, Lawrence Berkeley National Laboratory, Emeryville, CA, USA.

出版信息

Bioresour Technol. 2010 Jul;101(13):4900-6. doi: 10.1016/j.biortech.2009.10.066. Epub 2009 Nov 30.

DOI:10.1016/j.biortech.2009.10.066

PMID:19945861

Abstract

The efficiency of two biomass pretreatment technologies, dilute acid hydrolysis and dissolution in an ionic liquid, are compared in terms of delignification, saccharification efficiency and saccharide yields with switchgrass serving as a model bioenergy crop. When subject to ionic liquid pretreatment (dissolution and precipitation of cellulose by anti-solvent) switchgrass exhibited reduced cellulose crystallinity, increased surface area, and decreased lignin content compared to dilute acid pretreatment. Pretreated material was characterized by powder X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy, Raman spectroscopy and chemistry methods. Ionic liquid pretreatment enabled a significant enhancement in the rate of enzyme hydrolysis of the cellulose component of switchgrass, with a rate increase of 16.7-fold, and a glucan yield of 96.0% obtained in 24h. These results indicate that ionic liquid pretreatment may offer unique advantages when compared to the dilute acid pretreatment process for switchgrass. However, the cost of the ionic liquid process must also be taken into consideration.

摘要

比较了两种生物质预处理技术（稀酸水解和在离子液体中的溶解）在脱木质素、糖化效率和糖产率方面的效率，以柳枝稷作为模型生物能源作物。与稀酸预处理相比，离子液体预处理（纤维素通过反溶剂溶解和沉淀）后，柳枝稷的纤维素结晶度降低，比表面积增加，木质素含量降低。预处理后的材料通过粉末 X 射线衍射、扫描电子显微镜、傅里叶变换红外光谱、拉曼光谱和化学方法进行了表征。离子液体预处理显著提高了柳枝稷纤维素组分的酶水解速率，酶水解速率提高了 16.7 倍，24 小时内获得了 96.0%的葡萄糖产率。这些结果表明，与稀酸预处理过程相比，离子液体预处理可能为柳枝稷提供独特的优势。然而，也必须考虑到离子液体工艺的成本。