竹材的纤维素溶剂基生物质预处理，然后在超低纤维素酶用量下进行酶水解。

Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings.

机构信息

Biological Systems Engineering Department, Virginia Polytechnic Institute and State University, 210-A Seitz Hall, Blacksburg, VA 24061, USA.

出版信息

Bioresour Technol. 2010 Jul;101(13):4926-9. doi: 10.1016/j.biortech.2009.09.081. Epub 2009 Oct 23.

DOI:10.1016/j.biortech.2009.09.081

PMID:19854047

Abstract

The modified cellulose solvent- (concentrated phosphoric acid) and organic solvent- (95% ethanol) based lignocellulose fractionation (COSLIF) was applied to a naturally-dry moso bamboo sample. The biomass dissolution conditions were 50 degrees C, 1 atm for 60 min. Glucan digestibility was 88.2% at an ultra-low cellulase loading of one filter paper unit per gram of glucan. The overall glucose and xylose yields were 86.0% and 82.6%, respectively. COSLIF efficiently destructed bamboo's fibril structure, resulting in a approximately 33-fold increase in cellulose accessibility to cellulase (CAC) from 0.27 to 9.14 m(2) per gram of biomass. Cost analysis indicated that a 15-fold decrease in use of costly cellulase would be of importance to decrease overall costs of biomass saccharification when cellulase costs are higher than $0.15 per gallon of cellulosic ethanol.

摘要

改性纤维素溶剂（浓磷酸）和有机溶剂（95%乙醇）基木质纤维素分级（COSLIF）被应用于天然干燥的毛竹样品。生物质溶解条件为 50°C，1 大气压 60 分钟。在极低的纤维素酶用量（每克葡聚糖 1 个滤纸单位）下，葡聚糖的消化率为 88.2%。总的葡萄糖和木糖得率分别为 86.0%和 82.6%。COSLIF 有效地破坏了竹子的纤维结构，使纤维素对纤维素酶的可及性（CAC）从每克生物质 0.27 平方米增加到 9.14 平方米，增加了约 33 倍。成本分析表明，当纤维素酶成本高于每加仑纤维素乙醇 0.15 美元时，使用昂贵的纤维素酶减少 15 倍将有助于降低生物质糖化的总成本。