地氟醚预处理对糖尿病患者右房心肌的保护作用

Desflurane-induced postconditioning of diabetic human right atrial myocardium in vitro.

机构信息

Laboratoire d'anesthésiologie expérimentale et de physiologie cellulaire EA 3212, service d'anesthésie réanimation, institut fédératif de recherche ICORE 146, université de Caen, Basse Normandie, avenue Côte-de-Nacre, Caen cedex, France.

出版信息

Diabetes Metab. 2010 Feb;36(1):21-8. doi: 10.1016/j.diabet.2009.06.006. Epub 2009 Nov 28.

DOI:10.1016/j.diabet.2009.06.006

PMID:19945895

Abstract

AIM

We tested the hypothesis that brief exposure to desflurane at the time of reoxygenation might be able to protect against hypoxia-reoxygenation injury in human myocardium from diabetic (insulin-dependent, ID; and non-insulin-dependent, NID) patients and non-diabetic (ND) subjects.

METHODS

The force of contraction (34 degrees C, stimulation frequency 1Hz) in the right atrial trabeculae was recorded during 30min of hypoxia followed by 60min of reoxygenation. Desflurane (at 3, 6 and 9%) was administered during the first 5min of reoxygenation. The force of contraction at the end of the 60-min reoxygenation period (FoC(60)) was compared in the study groups (means+/-SD).

RESULTS

In the ND group, desflurane at 3, 6 and 9% (FoC(60): respectively 78+/-10%, 84+/-4% and 85+/-12% of baseline) enhanced the recovery of FoC(60) compared with the ND-controls (53+/-7% of baseline; P<0.05). In the ID group, desflurane at 3% (61+/-4%) did not modify the recovery of FoC(60) compared with the ID-controls (54+/-6%), whereas desflurane at 6 and 9% (75+/-11% and 81+/-8%, respectively) enhanced the recovery of FoC(60)vs the controls (P<0.05). In the NID group, desflurane at 3% (57+/-5%) also failed to modify the recovery of FoC(60) compared with the NID-controls (52+/-10%), while desflurane at 6 and 9% (80+/-10% and 79+/-7%, respectively) enhanced the recovery of FoC(60)vs the controls (P<0.05).

CONCLUSION

Desflurane in vitro was able to postcondition diabetic (both ID and NID) human myocardium at 6 and 9%, but not at 3%.

摘要

目的

我们检验了这样一个假说，即在复氧时短暂接触地氟醚可能有助于防止来自糖尿病（胰岛素依赖型，ID；和非胰岛素依赖型，NID）患者和非糖尿病（ND）个体的心肌缺氧/复氧损伤。

方法

在 34°C，刺激频率 1Hz 的条件下，记录 30 分钟缺氧后 60 分钟复氧期间右心房小梁的收缩力（张力）。在复氧的前 5 分钟给予地氟醚（3%、6%和 9%）。比较研究组在 60 分钟复氧期末的收缩力（FoC(60)）（平均值±SD）。

结果

在 ND 组中，地氟醚在 3%、6%和 9%（FoC(60)：分别为基础值的 78±10%、84±4%和 85±12%）增强了 FoC(60)的恢复，与 ND 对照组（基础值的 53±7%；P<0.05）相比。在 ID 组中，地氟醚在 3%（61±4%）时，与 ID 对照组（54±6%）相比，并未改变 FoC(60)的恢复，而地氟醚在 6%和 9%（75±11%和 81±8%）时，增强了 FoC(60)的恢复，与对照组相比（P<0.05）。在 NID 组中，地氟醚在 3%（57±5%）时也未能改变 FoC(60)的恢复，与 NID 对照组（52±10%）相比，而地氟醚在 6%和 9%（80±10%和 79±7%）时，增强了 FoC(60)的恢复，与对照组相比（P<0.05）。