Fan Tingjun, Wang Dansheng, Fu Yongfeng, Zhao Jun, Cong Rishan, Yang Xiuxia, Wang Jing
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Oct;26(5):1016-20.
Un-transfected rabbit corneal endothelial cells (RCECs) were cultivated, using chitosan blend membrane 4ha (chitosan-hyaluronic acid), 631ha (chitosan-hyaluronic acid) and 631s (chitosan-chondroitine sulfate) as scaffold carriers. Their biocompatibilities were studied in regard to cell adherence, morphological changes, growth status and monolayer forming abilities. The results indicated that RCECs cultivated on 4ha and 631ha carriers tended to be aggregated and even desquamated to some extent in local areas, and even more severely on 631ha carrier. And the RCECs cultivated on 631ha carrier could form almost a monolayer 48h later, and those on 4ha carrier could not. Contrarily, the RCECs cultivated on 631s carrier were evenly distributed and were in good status of growth with a good adherence and fibroblast-like morphology which could form almost a monolayer 48h later. And a complete monolayer was formed and was tightly attached to the 631s carrier 72h later. From the above results, it can be concluded that 631s carrier is most probably an ideal scaffold carrier for RCEC cultivation. 631s carrier may have the potential for use in the development of tissue-engineered rabbit corneal endothelium.
使用壳聚糖共混膜4ha（壳聚糖-透明质酸）、631ha（壳聚糖-透明质酸）和631s（壳聚糖-硫酸软骨素）作为支架载体，培养未转染的兔角膜内皮细胞（RCECs）。从细胞黏附、形态变化、生长状态和单层形成能力方面研究了它们的生物相容性。结果表明，在4ha和631ha载体上培养的RCECs有聚集倾向，局部甚至有一定程度的脱屑，在631ha载体上更为严重。在631ha载体上培养的RCECs在48小时后几乎可形成单层，而在4ha载体上培养的则不能。相反，在631s载体上培养的RCECs分布均匀，生长状态良好，黏附性好，呈成纤维细胞样形态，48小时后几乎可形成单层。72小时后形成完整单层并紧密附着于631s载体。从上述结果可以得出结论，631s载体很可能是用于培养RCEC的理想支架载体。631s载体可能有潜力用于组织工程化兔角膜内皮的开发。
