College of Marine Life Sciences, Ocean University of China, Qingdao, China.
J Mater Sci Mater Med. 2011 Jan;22(1):175-83. doi: 10.1007/s10856-010-4190-6. Epub 2010 Nov 25.
A novel chitosan-based membrane that made of hydroxyethyl chitosan, gelatin and chondroitin sulfate was used as a carrier of corneal endothelial cells. The characteristics of the blend membrane including transparency, equilibrium water content, ion and glucose permeability were determined. The results showed that the optical transparency of the membrane was as good as the natural human cornea. The water content of this scaffold was 81.32% which was remarkably close to the native cornea. The membrane had a good ion permeability and its glucose permeability was even higher than natural human cornea. The cultured rabbit corneal endothelial cells formed a monolayer on the membrane. The results demonstrated that the membrane was suitable for corneal endothelial cells to attach and grow on it. In addition, the membranes in vivo could be degraded steadily with less inflammation and showed a good histocompatibility. These results demonstrated that the hydroxyethyl chitosan-chondroitin sulfate-gelatin blend membrane can potentially be used as a carrier for corneal endothelial cell transplantation.
一种新型的壳聚糖基膜，由羟乙基壳聚糖、明胶和硫酸软骨素组成，被用作角膜内皮细胞的载体。该共混膜的特性，包括透明度、平衡含水量、离子和葡萄糖渗透性都进行了测定。结果表明，该膜的光学透明度与天然人眼角膜相当。该支架的含水量为 81.32%，与天然角膜非常接近。该膜具有良好的离子渗透性，其葡萄糖渗透性甚至高于天然人眼角膜。培养的兔角膜内皮细胞在膜上形成单层。结果表明，该膜适合角膜内皮细胞附着和生长。此外，体内的膜可以稳定降解，炎症反应较少，具有良好的组织相容性。这些结果表明，羟乙基壳聚糖-软骨素-明胶共混膜有可能作为角膜内皮细胞移植的载体。
