Song Yulin, Zheng Qixin, Zheng Jianfeng
Department of Orthopaedics, Second Affiliated Hospital, Nanchang University, Nanchang 330006, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Oct;26(5):1056-9.
The RGD-containing peptide was used to modify the surface of porous PLGA-[ASP-PEG], and was incubated in the modified simulated body fluid (mSBF) for two weeks. The mineralization of PLGA-[ASP-PEG] was explored. The active peptide was used to modify PLGA-[ASP-PEG] through cross-linker (Sulfo-LC-SPDP), characterized by X-ray photoelectron spectroscopy (XPS) the peptide-modified PLGA-[ASP-PEG] (Experiment group, EG) and PLGA-[ASP-PEG] without modification (Control group, CG) were all incubated in mSBF for two weeks, confirmed by observation of Scanning electron microscope(SEM) and measurements of Energy dispersive analysis system of X-ray (EDS) and X-ray diffractometry (XRD). XPS indicated that the binding energy of sulphur in EG was 164eV, and the ratio of carbon to sulphur in EG was 99.746 : 0.1014, however, sulphur was not detected in CG; SEM analysis demonstrated that the mineralization layers were more consecutive and compact in EG than in CG. The results of EDS and XRD indicated that the main component of mineral was hydroxyapatite, and the ratio of Ca/P was 1.60 in EG, and 1.52 in CG. RGD-containing peptide provided enough functional groups for mineralization; the mineralized peptide- modified PLGA-[ASP-PEG] possessed the bonelike microstructure.
含RGD的肽用于修饰多孔PLGA-[ASP-PEG]的表面，并在修饰后的模拟体液（mSBF）中孵育两周。探究了PLGA-[ASP-PEG]的矿化情况。活性肽通过交联剂（磺基-LC-SPDP）用于修饰PLGA-[ASP-PEG]，通过X射线光电子能谱（XPS）进行表征。将肽修饰的PLGA-[ASP-PEG]（实验组，EG）和未修饰的PLGA-[ASP-PEG]（对照组，CG）均在mSBF中孵育两周，通过扫描电子显微镜（SEM）观察、X射线能谱分析系统（EDS）测量和X射线衍射仪（XRD）进行确认。XPS表明，EG中硫的结合能为164eV，EG中碳与硫的比例为99.746:0.1014，然而，CG中未检测到硫；SEM分析表明，EG中的矿化层比CG中的更连续、更致密。EDS和XRD的结果表明，矿物质的主要成分是羟基磷灰石，EG中Ca/P的比例为1.60，CG中为1.52。含RGD的肽为矿化提供了足够的官能团；矿化的肽修饰PLGA-[ASP-PEG]具有类骨微观结构。
