Department of Pulmonary Medicine, Research Institute of Respiratory Disease, Fudan University, Zhongshan Hospital, Shanghai, China.
Respirology. 2010 Jan;15(1):99-106. doi: 10.1111/j.1440-1843.2009.01667.x. Epub 2009 Nov 30.
Continuous monitoring of PaO(2) in seriously ill patients is an important aspect of clinical management, especially for patients with acute lung injury (ALI) or acute respiratory distress syndrome. We have developed a fibreoptic sensor to detect PaO(2)in vivo based on fluorescence quenching technology. In this study we evaluated the sensitivity of this sensor in monitoring PaO(2) in a rabbit model with ALI.
The oxygen sensor is a membrane made of Ru(dpp)(3)(PF6)(2), poly-2-methacryloyloxyethyl phosphorylcholine and butylmethacylate copolymer p-(MPC-co-BMA) located at the tip of the optical fibre. The sensor was inserted into the carotid artery of the animals and monitored PaO(2) continuously. Oleic acid was intravenously injected to induce lung injury. Simultaneous comparisons were made between PaO(2) measured by blood gas analysis and PaO(2) measured by the fibreoptic sensor, both before and after lung injury.
The fluorescence intensity decreased gradually following ALI, reflecting increasing hypoxia. Correlation coefficients between measurements by the oxygen sensor and by the blood gas analysis were 0.995 +/- 0.003, 0.994 +/- 0.002 and 0.993 +/- 0.005 (P < 0.05) for control animals, animals with ALI and animals with electrolyte disturbance, respectively. The bias and precision for normal animals was -1.5 +/- 10.8 mm Hg, for animals with ALI was -1.2 +/- 11.2 mm Hg and for animals with electrolyte disturbance was -1.4 +/- 9.2 mm Hg.
The oxygen sensor showed high accuracy and stability for continuous monitoring of PaO(2) in normal animals, in animals with ALI and in animals with electrolyte disturbance, suggesting that it may be clinically useful in the continuous measurement of oxygen partial pressure.
持续监测重病患者的 PaO₂是临床管理的重要方面，特别是对急性肺损伤（ALI）或急性呼吸窘迫综合征患者。我们基于荧光猝灭技术开发了一种光纤传感器来检测体内的 PaO₂。在这项研究中，我们评估了该传感器在监测兔 ALI 模型中 PaO₂的敏感性。
氧传感器是位于光纤尖端的 Ru(dpp)(3)(PF6)(2)、聚-2-甲基丙烯酰氧乙基磷酸胆碱和丁基甲基丙烯酸酯共聚物 p-(MPC-co-BMA)组成的膜。将传感器插入动物的颈动脉中，连续监测 PaO₂。通过静脉注射油酸诱导肺损伤。在肺损伤前后，分别通过血气分析和光纤传感器同时比较 PaO₂的测量值。
ALI 后荧光强度逐渐降低，反映出缺氧增加。氧传感器和血气分析之间的相关系数分别为 0.995 ± 0.003、0.994 ± 0.002 和 0.993 ± 0.005（P < 0.05），用于对照动物、ALI 动物和电解质紊乱动物。正常动物的偏差和精度为-1.5 ± 10.8mmHg，ALI 动物为-1.2 ± 11.2mmHg，电解质紊乱动物为-1.4 ± 9.2mmHg。
氧传感器在正常动物、ALI 动物和电解质紊乱动物中对 PaO₂的连续监测表现出高精度和稳定性，表明它在氧分压的连续测量中可能具有临床应用价值。
