Department of Materials Science and Engineering, Yonsei University, 134 Shin-chon-dong, Seo-dae-mun-gu, Seoul, 120-749, Republic of Korea.
J Immunol Methods. 2010 Feb 28;353(1-2):44-8. doi: 10.1016/j.jim.2009.11.015. Epub 2009 Dec 3.
Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400-500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.
各种类型的聚苯乙烯微孔板已被广泛用于免疫分析。通过在微孔板上热沉积氨基修饰的对二甲苯（parylene-A），开发了一种新的适合蛋白质和肽共价固定化的微孔板。利用 parylene-A 的伯胺基团进行蛋白质和肽的共价偶联。在 400-500nm 波长处的光透过率估计适合于免疫分析的应用。通过使用辣根过氧化物酶（HRP）、抗 HRP 抗体和 9 个残基的肽作为模型生物分子，证明涂覆有 parylene-A 的微孔板的固定化效率远远优于传统微孔板。
