Key Laboratory of Applied Surface and Colloid Chemistry (Shaanxi Normal University), School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, China.
Anal Bioanal Chem. 2011 Oct;401(7):2311-7. doi: 10.1007/s00216-011-5306-7. Epub 2011 Aug 14.
Microplates made of polystyrene have been widely used for immunoassays. Protein molecules that have been immobilized on a hydrophobic polystyrene microplate by passive adsorption lose their activity and suffer considerable denaturation. A new chitosan-coated microplate suitable for the covalent immobilization of enzymes has been developed. The primary amino groups of chitosan were exploited for this covalent coupling of proteins. The optical transmittance of the chitosan-coated microplate, at wavelengths of 400-800 nm, was estimated to be suitable for its application in chromogenic reaction-based bioassays. The immobilization efficiency of the chitosan-coated microplate was demonstrated to be far superior to that of a conventional microplate when tested using acetylcholinesterase (AChE) and β-glucosidase as model biomolecules, and the chitosan-coated microplate may thus have potential applications in biosensing and bioreactor systems.
聚苯乙烯微孔板已被广泛应用于免疫测定。通过被动吸附固定在疏水性聚苯乙烯微孔板上的蛋白质分子会失去活性并发生严重变性。一种新的壳聚糖涂覆的微孔板已经被开发出来，它适用于酶的共价固定化。壳聚糖的伯氨基被用于蛋白质的这种共价偶联。壳聚糖涂覆的微孔板在 400-800nm 波长处的光透射率估计适用于基于比色反应的生物测定。当使用乙酰胆碱酯酶（AChE）和β-葡萄糖苷酶作为模型生物分子进行测试时，壳聚糖涂覆的微孔板的固定效率被证明远远优于传统的微孔板，因此壳聚糖涂覆的微孔板可能在生物传感和生物反应器系统中有潜在的应用。
