Departamento de Microbiología, Facultad de Farmacia, Granada, Spain.
Bioresour Technol. 2010 Apr;101(7):2096-105. doi: 10.1016/j.biortech.2009.10.091. Epub 2009 Nov 30.
A cultivation independent approach (PCR-TGGE) was used to evaluate the occurrence of Archaea in four wastewater treatments based on technologies other than activated sludge, and to comparatively analyze their community structure. TGGE fingerprints (based on partial archaeal 16S-rRNA amplicons) were obtained from sludge samples taken from a pilot-scale aerated MBR fed with urban wastewater and operated under two different sets of conditions (MBR1 and MBR2 treatments), and also from biofilms sampled from two pilot-scale submerged biofilters (SBs) consisting of one aerated and one anoxic column each, fed with urban (USB treatment) or industrial (ISB treatment) wastewater, respectively. Analysis of TGGE fingerprints revealed clear and significant differences of the community structure of Archaea between the wastewater treatments studied, primarily according to wastewater origin and the type of technology. Thirty-two different band classes were detected among the 23 sludge and biofilm samples analyzed, from which five were selected as dominant or distinctive of the four treatments studied. Sixteen predominant TGGE bands were identified, revealing that all of them were related to methanogenic Archaea. Neither other Euryarchaeota groups nor Crenarchaeota members were identified amongst the 16S-rRNA fragments sequenced from separated TGGE bands.
采用培养独立性方法（PCR-TGGE）评估了基于除活性污泥以外的技术的 4 种废水处理中古菌的出现，并比较分析了它们的群落结构。从采用城市废水并在两种不同条件下运行的中试规模曝气膜生物反应器（MBR1 和 MBR2 处理）中的污泥样品和由每个各有氧和缺氧柱组成的两个中试规模淹没生物滤池（SB）中的生物膜中获得了基于部分古菌 16S-rRNA 扩增子的 TGGE 指纹图谱（SBs），分别用城市废水（USB 处理）和工业废水（ISB 处理）进料。TGGE 指纹图谱分析表明，所研究的废水处理之间古菌群落结构存在明显而显著的差异，主要取决于废水来源和技术类型。在所分析的 23 个污泥和生物膜样品中检测到 32 种不同的条带类，其中 5 种被选为 4 种处理的优势或特征条带。鉴定出 16 个主要的 TGGE 条带，揭示它们均与产甲烷古菌有关。从分离的 TGGE 条带中测序的 16S-rRNA 片段中未鉴定出其他广古菌组或泉古菌门成员。
