比较检测聚酯背 Polyacrylamide 凝胶中 DNA 的银染方案。
A comparison of silver staining protocols for detecting DNA in polyester-backed polyacrylamide gel.
机构信息
Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences , Fuzhou 350003 , China.
出版信息
Braz J Microbiol. 2012 Apr;43(2):649-52. doi: 10.1590/S1517-83822012000200029. Epub 2012 Jun 1.
Abstract
Eight silver-staining protocols were applied to detect DNA in polyester-backed gels to select the optimal. Results showed important differences in staining quality and that four methods were well-suited for TGGE gels due to high sensitivity and low background, including the Bassam et al. methods, the manufacturer method and our improved method.
摘要
八种银染方案被应用于聚酯背胶凝胶中以检测 DNA，从而选择出最佳方案。结果显示出染色质量的重要差异，其中四种方法由于高灵敏度和低背景而非常适合 TGGE 凝胶，包括 Bassam 等人的方法、制造商的方法和我们改进的方法。
Zotero 插件