产甲烷八叠球菌中依赖于铁氧还蛋白的膜结合电子传递中 Ech 氢化酶的功能。
Function of Ech hydrogenase in ferredoxin-dependent, membrane-bound electron transport in Methanosarcina mazei.
机构信息
Institute of Microbiology and Biotechnology, University of Bonn, Meckenheimer Allee 168, 53115 Bonn, Germany.
出版信息
J Bacteriol. 2010 Feb;192(3):674-8. doi: 10.1128/JB.01307-09. Epub 2009 Nov 30.
Abstract
Reduced ferredoxin is an intermediate in the methylotrophic and aceticlastic pathway of methanogenesis and donates electrons to membrane-integral proteins, which transfer electrons to the heterodisulfide reductase. A ferredoxin interaction has been observed previously for the Ech hydrogenase. Here we present a detailed analysis of a Methanosarcina mazei Delta ech mutant which shows decreased ferredoxin-dependent membrane-bound electron transport activity, a lower growth rate, and faster substrate consumption. Evidence is presented that a second protein whose identity is unknown oxidizes reduced ferredoxin, indicating an involvement in methanogenesis from methylated C(1) compounds.
摘要
还原型铁氧还蛋白是甲基营养型和乙酸营养型产甲烷途径中的一种中间产物，它将电子捐赠给膜整合蛋白，这些蛋白再将电子传递给异二硫键还原酶。先前已经观察到 Ech 氢化酶与铁氧还蛋白相互作用。在这里，我们对 Methanosarcina mazei Delta ech 突变体进行了详细分析，结果表明该突变体的还原型铁氧还蛋白依赖性膜结合电子传递活性降低，生长速度较慢，底物消耗速度较快。有证据表明，一种身份不明的第二种蛋白质氧化还原型铁氧还蛋白，表明其参与了甲基化 C(1)化合物的甲烷生成。
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