利用绿色荧光蛋白监测乳球菌细胞包膜应激。
Use of green fluorescent protein to monitor cell envelope stress in Lactococcus lactis.
机构信息
Department of Technology and Biotechnology of Dairy Products, Instituto de Productos Lácteos de Asturias, IPLA-CSIC, Apdo. 85, 33300 Villaviciosa, Asturias, Spain.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):978-81. doi: 10.1128/AEM.02177-09. Epub 2009 Nov 30.
Abstract
A Lactococcus lactis reporter system suitable to detect cell envelope stress in high-throughput settings was developed by fusing the CesR-regulated promoter of llmg0169 to the gfp(uv) gene. A dot blot assay allowed fast detection of green fluorescent protein (GFP) fluorescence even at low production levels. Unexpectedly, this promoter was also induced by mitomycin C via CesR.
摘要
我们构建了一个适用于高通量筛选的乳球菌报告系统，该系统通过将 llmg0169 的 CesR 调控启动子与 gfp(uv)基因融合，实现了对细胞包膜应激的检测。点印迹实验允许即使在低产量水平下也能快速检测到绿色荧光蛋白（GFP）的荧光。出乎意料的是，该启动子也可被丝裂霉素 C 通过 CesR 诱导。
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