Division of Microbiology, United States Food and Drug Administration, College Park, Maryland 20740, USA.
Appl Environ Microbiol. 2010 Feb;76(3):945-7. doi: 10.1128/AEM.02046-09. Epub 2009 Nov 30.
The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS629, was observed within its gne gene. trans complementation with a functional gne gene from O157:H7 restored O157 antigen expression in MA6.
我们研究了 MA6（一株 O 粗糙：H7 志贺毒素大肠杆菌）的 O 抗原（rfb）操纵子和相关基因，以确定 O157 表达缺失的原因。在其 gne 基因内观察到一个 1310bp 的插入片段，与 IS629 同源。从 O157：H7 中用功能正常的 gne 基因进行转互补恢复了 MA6 中的 O157 抗原表达。
