基于转录组分析的大肠杆菌细胞表面脂肪酶的增强表达。
Enhanced display of lipase on the Escherichia coli cell surface, based on transcriptome analysis.
机构信息
Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Dongyang University, Yeongju, Gyeongbuk 750-711, Republic of Korea.
出版信息
Appl Environ Microbiol. 2010 Feb;76(3):971-3. doi: 10.1128/AEM.02463-09. Epub 2009 Nov 30.
Abstract
A cell surface display system was developed using Escherichia coli OmpC as an anchoring motif. The fused Pseudomonas fluorescens SIK W1 lipase was successfully displayed on the surface of E. coli cells, and the lipase activity could be enhanced by the coexpression of the gadBC genes identified by transcriptome analysis.
摘要
利用大肠杆菌 OmpC 作为锚定基序，开发了一种细胞表面展示系统。融合的荧光假单胞菌 SIK W1 脂肪酶成功地展示在大肠杆菌细胞表面，并且通过转录组分析鉴定的 gadBC 基因的共表达可以增强脂肪酶活性。
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