Department of Pediatrics, Division of Neonatology, Wake Forest University Health Sciences, Winston-Salem, NC 27157, USA.
J Pediatr Gastroenterol Nutr. 2010 Jan;50(1):16-21. doi: 10.1097/MPG.0b013e3181c2c2af.
Few infant animal models have been used to study infantile short bowel syndrome (SBS). Most SBS models involve removal of the proximal small bowel followed by jejunoileal anastomosis, which has unclear clinical relevance to human infantile SBS that often results from surgical treatment for necrotizing enterocolitis and involves removal of the ileum, ileocecal valve, and part of or the entire colon. Our objective was to develop a more appropriate SBS model in developing rats.
Twenty-day-old weanling rats were divided into 2 surgery groups, ileocecal resection (ICR) and sham groups, and a control group that did not undergo surgery. All were fed a liquid diet ad libitum for 7 days after surgery or for 7 days in the controls, and body weight, food intake, and stool changes were recorded daily. The rats were then euthanized and intestinal lengths and weights were recorded. Samples of intestine from the distal jejunum and proximal colon were collected for histology. Mucosal samples from the middle, distal jejunum, and colon were collected for measurements of mucosal weights, DNA, RNA, and protein levels. Maltase activity was determined in the small intestine.
Eighty-five percent of rats survived the ICR with subsequent development of diarrhea, hyperphagia, and poor growth. Adaptive responses to ICR, as compared with sham, were evidenced by increased intestinal and mucosal weights, DNA, RNA, and protein levels, increased maltase activity and villous thickness in distal jejunum, and increased mucosal thickness in the colon.
This ICR model in weanling rats is appropriate for studying human infantile SBS.
用于研究婴儿短肠综合征（SBS）的幼年动物模型较少。大多数 SBS 模型涉及近端小肠的切除，然后进行空肠回肠吻合术，但这与人类婴儿 SBS 的临床相关性并不明确，因为后者通常是由坏死性小肠结肠炎的手术治疗引起的，涉及回肠、回盲瓣和部分或整个结肠的切除。我们的目的是在发育中的大鼠中建立一种更合适的 SBS 模型。
20 天大的断奶大鼠被分为 2 组手术组（回盲切除术 ICR 组和假手术组）和 1 组对照组（未接受手术）。手术后，所有大鼠均自由摄入液体饮食 7 天，或对照组大鼠不进行手术，摄入液体饮食 7 天。每天记录体重、食物摄入量和粪便变化。然后处死大鼠并记录肠道长度和重量。收集远端空肠和近端结肠的肠段样本进行组织学检查。从中段、远端空肠和结肠采集黏膜样本，用于测量黏膜重量、DNA、RNA 和蛋白质水平。测定小肠中的麦芽糖酶活性。
85%的 ICR 大鼠术后存活，随后出现腹泻、食欲亢进和生长不良。与假手术相比，ICR 后的适应性反应表现为肠道和黏膜重量、DNA、RNA 和蛋白质水平增加，远端空肠麦芽糖酶活性和绒毛厚度增加，以及结肠黏膜厚度增加。
这种在断奶大鼠中的回盲切除术 ICR 模型适合用于研究人类婴儿 SBS。
