短肠综合征生长型和成熟型机体模型的生长适应过程是否存在差异？
Are there differences in the growth adaptation processes of growing and mature organism models of short bowel syndrome?
作者信息
Tannuri Ana Cristina Aoun, Rotondo Ĺtalo Geraldo, Barros Guilherme Garcia, Vaisberg Victor Van, Mendes-Neto Cícero, Paes Vitor Ribeiro, Coelho Maria Cecilia Mendonça, Gonçalves Josiane, Serafini Suellen, Tannuri Uenis
机构信息
Divisao de Cirurgia Pediatrica, Unidade Pediatrica de Transplante de Figado e Laboratorio de Pesquisa em Cirurgia Pediatrica (LIM 30), Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR.
出版信息
Clinics (Sao Paulo). 2018 Oct 18;73:e499. doi: 10.6061/clinics/2018/e499.
OBJECTIVES
The purpose of this study was to present an experimental model of short bowel syndrome (SBS) in weaning rats and to compare the adaptative mechanisms of the remaining bowel in weaning rats and adult animals by means of morphometric, histologic and molecular methods.
METHODS
Twenty-four weaning rats were divided into 3 groups of 8 animals, one control group and two short bowel groups (euthanasia after 4 and 21 days), and were compared with similar adult groups. Morphometric evaluations of the animals and histopathological and molecular studies of the remaining bowel were performed.
RESULTS
The weight of young rats increased after enterectomy, whereas that of adult rats decreased after enterectomy (p<0.0001). The ratio of intestinal length/body weight was significantly higher in weaning rats than in adults (p<0.002), showing that intestinal growth was more intense in weaning rats. Intestinal resection promoted increased thickness of the small bowel lamina propria (p=0.001) and reduced thickness of the colon lamina propria (p=0.04) in weaning rats relative to those in adults. In addition, intestinal resection promoted increased expression of the Bcl-xl gene (antiapoptotic) in adult animals compared with that in weaning rats (p=0.001).
CONCLUSION
Morphometric, histological and molecular differences were shown in the adaptation processes of growing and mature organisms.
目的
本研究旨在建立断奶大鼠短肠综合征（SBS）的实验模型，并通过形态学、组织学和分子学方法比较断奶大鼠和成年动物剩余肠段的适应性机制。
方法
将24只断奶大鼠分为3组，每组8只，一组为对照组，两组为短肠组（分别于术后4天和21天安乐死），并与相似的成年组进行比较。对动物进行形态学评估，并对剩余肠段进行组织病理学和分子学研究。
结果
幼鼠肠切除术后体重增加，而成鼠肠切除术后体重下降（p<0.0001）。断奶大鼠的肠长/体重比显著高于成年大鼠（p<0.002），表明断奶大鼠的肠道生长更为活跃。与成年大鼠相比，断奶大鼠肠切除术后小肠固有层厚度增加（p=0.001），结肠固有层厚度减少（p=0.04）。此外，与断奶大鼠相比，成年动物肠切除术后促凋亡基因Bcl-xl的表达增加（p=0.001）。
结论
生长中的生物体和成熟生物体在适应过程中表现出形态学、组织学和分子学差异。
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