Wang Hao, Zhang Peng, Wang Chun-Ting
State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Sep;40(5):853-6.
To study on the expression pattern of a novel gene, Biot2-L, in mice and its primary bio-function on the development of testis.
Bioinformatics analysis were applied to identify the structure characteristics and homologene of Biot2-L. RT-PCR and Real-time RT-PCR were used to analyze its expression profile in normal tissues and the time-dependent expression pattern during the testes sexual maturation.
The complete sequence of Biot2-L was obtained. A conserved domain-CCDC7 was detected and there were homolog proteins in advanced mammals with about 50% identity by bioinformatics analysis. The highly expression of Biot2-L in adult testis but low or absent expressed in other tissues was observed. A regular change of Biot2-L expression level during the testis development and spermatogonia was demonstrated by the results of Real-time RT-PCR. The mRNA transcript appeared from the fifth week of postnatal development, and the expression level increased up to adulthood, but decreased when get older.
Biot2-L was highly expressed in adult testis and regular changed during the testis development, which suggested that the Biot2-L would be involved in the testis development and spermatogonia.
研究新基因Biot2-L在小鼠中的表达模式及其对睾丸发育的主要生物学功能。
应用生物信息学分析鉴定Biot2-L的结构特征和同源基因。采用RT-PCR和实时RT-PCR分析其在正常组织中的表达谱以及睾丸性成熟过程中的时间依赖性表达模式。
获得了Biot2-L的完整序列。通过生物信息学分析检测到一个保守结构域CCDC7，在高等哺乳动物中存在同源蛋白，同源性约为50%。观察到Biot2-L在成年睾丸中高表达，而在其他组织中低表达或不表达。实时RT-PCR结果显示，Biot2-L在睾丸发育和精原细胞过程中表达水平有规律变化。mRNA转录本在出生后第5周出现，表达水平在成年期升高，但随着年龄增长而降低。
Biot2-L在成年睾丸中高表达，并在睾丸发育过程中有规律变化，提示Biot2-L可能参与睾丸发育和精原细胞过程。
